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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Methodology according to national guideline, but no analytical verification of test concentration and purity and source of compound not reported.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Isophamchloride
IUPAC Name:
Isophamchloride
Details on test material:
White powder, low solubility and experiation date of April 1993

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Test is conducted in 300 ml Erlenmeyer flasks on a mechanical shaker under an incubation hood. The inhibition in the control and test samples is measured in a UV-VIS spectrophotometer at a coating/film thickness of 10 mm in disposable cuvettes. Erlenmeyer flasks incubated at 21 (+/- 1) degrees C on a mechanical shaker under an incubation hood.

Test solutions

Vehicle:
no
Details on test solutions:
Test Concentrations: 100, 200, 300, 400 and 500 mg/l

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h

Test conditions

Test temperature:
21 degrees centigrade
Nominal and measured concentrations:
See table below.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
170 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
340 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
EC50 (h) 340 mg/l; EC10 (h) 170 mg/l . Inhibition values %: 100 mg/l - 5%; 200 mg/l - 14%; 300 mg/l - 19%; 400 mg/l - 66%; 500 mg/l 99%; EC10 and EC50 determined by linear regression .

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The cell proliferation test conducted using isophamchloride according to a national guideline indicates that the substance has an EC10 and EC50 of 170 and 340 mg/L, respectively.
Executive summary:

A DIN38412 cell proliferation inhibition test was conducted using psedomonas putida. The test was conducted according to a national guideline methodology but no analytical verification of test concentration and purity and source of compound not reported. The EC10 and EC50 were determined to be 170 and 340 mg/l, respectively.