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Acute tests evaluating the toxicity of 2-methyl-N-(4-sulfamoylphenyl) prop-2-enamide (SPM-N) to aquatic organisms from three different trophic levels are available. The substance did not cause any adverse effects on fish survival (LC50 (96 h) > 29 mg/L, measured), aquatic invertebrates mobility (EC50 (48 h)> 29 mg/L, measured) or algal growth (EC50 (48 h) > 30 mg/L). No significant effects were observed on the activity of activated sludge microorganisms after 3 hours of exposure, leading to an EC50 > 100 mg/L (nominal). This statement is supported by the results of the toxicity control in the biodegradation test (see IUCLID section 5.2.1) which showed that SPM-N is not inhibitory to aquatic microorganisms (35% biodegradation within 14 days).

Based on the available information, SPM-N is considered to be not acutely toxic to aquatic organisms up to limit of its water solubility (30.3 mg/L).