Registration Dossier

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Title:
No information
Author:
Anonymous
Year:
2010
Bibliographic source:
Category approach for PI, Pam and their salts, 2010

Materials and methods

Test guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
static
Total exposure duration:
17 h

Results and discussion

Effect concentrations
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
ca. 140 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: light absorption at 436nm
Details on results:
See category for PI, PAM and their salts.

The toxicity to micro organisms was estimated by comparing with the measured toxicity (EC50) for PI, PDPI and PPI versus the weight percentage of PAM in each of the substances:

weight%       EC50 (mg/l)
PI              0        140
PDPI       46.5        293/270, resp. 360
PPI          63.5        140      
PAM       100        estimated

PI = 2-phenyl-2 -imidazoline, CAS 936-49 -2
PAM = benzene-1,2,4,5 -tetracarboxylic acid, CAS 89 -05 -4
PDPI = 1 mol PAM plus 2 mol PI, CAS 54553 -91 -2
PPI = 1 mol PAM plus 1 mol PI, CAS 54553 -90 -1

Applicant's summary and conclusion