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Ecotoxicological information

Toxicity to soil microorganisms

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Description of key information

The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 value of greater than 1000 mg/kg. The No Observed Effect Concentration (NOEC) after 28 days exposure was 1000 mg/kg (OECD 216 and EU Method C.21).

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GUIDELINE

Nitrogen transformation activity of soil microorganisms was investigated using a method designed to be compatible with OECD Guidelines for Testing of Chemicals (2000) No 216 "Soil Microorganisms: Nitrogen Transformation Test" and Method C.21 of Commission Regulation (EC) No. 440/2008.

METHODS

Soil microorganisms were exposed to the test item at concentrations of 10, 32, 100, 320 and 1000 mg/kg for 28 days at temperatures of between 19 and 21°C in the dark with the addition of powdered Lucerne-green-grass meal to act as a respiratory substrate.

The inhibitory effort of the test item on nitrogen transformation was assessed by the determination of nitrate concentration in the soil samples on Days 0 and 28 and compared to data obtained from control soil samples.

RESULTS

The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 value of greater than 1000 mg/kg. The No Observed Effect Concentration (NOEC) after 28 days exposure was 1000 mg/kg.