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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Acutely not harmful to fish (LL50 > 100 mg/L)

Key value for chemical safety assessment

Additional information

In the key study, fish acute toxicity was investigated under GLP conditions following OECD TG 203. Due to the low water solubility, the test concentrations were prepared as water accomodated fractions (WAF) as described in OECD TG 23. The test concentrations could not be chemically verifed due to the lack of a sufficiently sensitive method to determine the test substance in the test solutions up to its saturation limit. Therefore, the results are based on loading rate ("nominal concentrations"). No effects were found up to the highest tested concentrations. The 96 -h LL50 was > 100 mg/L (BASF, 2011).

A second guideline study is available: a static test with the Golden orfe (Leuciscus idus) which followed the German Industrial Standard DIN 38412, part 15. This study is of lower reliability than the above described study as it was reported that on the water surface an oily film was observed in all test concentrations. Moreover, after 96 h the aquaria were cloudy in the concentrations equal to or higher than 4.64 mg/L. Based on nominal results, the 96 -h LC50was determined to be 10.52 mg/L. The NOEC was 4.64 mg/L (BASF, 1987).

The results are further supported by two older studies which were performed similarily to nowadays methods with the analogous test substance Adogen 283 (di-iso-tridecylamine, CAS 57157 -80 -9). Based on nominal concentrations, the 4-d LC50 for effects on rainbow trout (reported as Salmo gairdneri) was determined to be > 10 mg/L (measured: > 500 µg/L; Dave & Lidman, 1978). The 96 -h LC50 for effects on zebrafish (reported as Brachydanio rerio) was given with > 5.6 mg/L (no mortality up to the highest concentration tested), based on nominal concentrations (Dave et al., 1981).

Results reported by Dave et al. (1979) regarding effects of Adogen 283 on rainbow trout (reported as Salmo gairdneri) were disregarded due to the poor documentation and the use of petroleum ether as vehicle. Two other Adogen 283 studies of Dave et al. (1981) with embryo and larval stages of rainbow trout and zebrafish were also not considered further as the test system is unsuitbale for covering this endpoint.