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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
wide range of treated area size

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
BASF Test: Before OECD guidelines were established, an internal standardized test was performed. 3 animals per sex per dose were treated for 24 h under occlusive conditions. Observations were performed on weekdays, weighing was performed several times during the observation period of 14 d.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
tert-butyl acrylate
EC Number:
216-768-7
EC Name:
tert-butyl acrylate
Cas Number:
1663-39-4
Molecular formula:
C7H12O2
IUPAC Name:
tert-butyl acrylate
Specific details on test material used for the study:
- Name of test material: Tertiaerbutylacrylat
- Physical state: clear liquid
- Analytical purity: ca. 99.5 %
- Impurities: stabilized with 15 ppm Hydrochinonmonomethylether

Test animals

Species:
rabbit
Strain:
Vienna White
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Gaukler, D
- Mean weight at study initiation: 2.85 kg (males); 2.92 kg (females)
- Diet (e.g. ad libitum): Ssniff

ENVIRONMENTAL CONDITIONS
not reported

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Area of exposure: 108-247 cm2, clipped back

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.4 or 2 g/kg bw.
- Constant concentration used: yes

REMOVAL OF TEST SUBSTANCE
- Washing (if done): Lutrol/ water
- Time after start of exposure: 24 h
Duration of exposure:
24 h
Doses:
400, 2000 mg/kg bw
No. of animals per sex per dose:
3
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7d (low dose)/ 14 d (high dose)
- Frequency of observations and weighing: observations on workdays, weighing at the beginning and the end of the test
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs- Duration of observation period following administration: 14 days (or other?)
- Frequency of observations and weighing:
- Necropsy of survivors performed: yes/no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 2 000 mg/kg bw
Mortality:
- 2000 mg/kg: 3/6 animals died
- 400 mg/kg: 0/6 animals died
see table for details
Clinical signs:
2000 mg/kg: apathy, accelerated respiration, screaming
Other findings:
Local effects: obvious primary irritation effects, which were not fully reversible within the observation period; scaling at the end of the observation period.

Any other information on results incl. tables

Cumulative mortality

Dose (mg/kg bw)

sex

after 1 h

after 24 h

after 48 h

after 7 d

after 14 d

2000

male

0/3

1/3

1/3

1/3

1/3

female

0/3

1/3

2/3

2/3

2/3

400

male

0/3

0/3

0/3

0/3

female

0/3

0/3

0/3

0/3

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria