2-amino-2-ethylpropanediol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 Apr - 14 Jun 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A stock solution of 100 g/L was prepared. Aliquots were transferred into test flasks in amounts resulting in a disolution series separated by a spacing factor of 2. To each flask 10 mL culture medium and 10 mL inoculum with a FNU of 50 at 436 nm are added. Flasks are filled up to a final volume of 100 mL.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: source: Deutsche Sammlung für Mikroorganismen (DSMZ, German Collection of Microorganisms and Cell Cultures)
- Preparation of inoculum for exposure: pre-culture grown for 7 h at 21 °C before inoculation. pre-culture diluted with sterile culture medium, resulting in a FNU (Formazine Nephelometric Units) of 50

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Remarks on exposure duration:

16 ± 1 h

Test temperature:

21 ± 1 °C

Nominal and measured concentrations:

nominal: 0, 125, 250, 500, 1000, and 2000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 300 mL Erlenmeyer flasks, 100 mL fill volume
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2x

Reference substance (positive control):

no

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

640 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Table 1: Measured extinction values

Concentration (mg/L)	Replicate 1	Replicate 2	Replicate 3	Median value
control	1.314	1.332	1.358	1.335
125	1.448	1.355	1.351	1.385
250	1.362	1.401	1.309	1.357
500	0.901	0.949	0.912	0.921
1000	0.135	0.142	0.135	0.137
2000	0.031	0.026	0.024	0.027

Table 2: Inhibition values

Concentration (mg/L)	% inhibition of cell growth
125	-4
250	-2
500	32
1000	92
2000	100

Description of key information

EC10 = 300 mg/L with Pseudomonas putida (DIN 38412, part 8)
EC50 = 640 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:

640 mg/L

EC10 or NOEC for microorganisms:

300 mg/L

Additional information

There is one microorganism study available for 2-amino-2-ethyl-1,3-propanediol (AEPD), which was used as key study for the assessment. The study was conducted according to the guideline DIN 38412, part 8 (Schopka, 1991). A culture of the bacterium Pseudomonas putida was exposed to AEPD for 16 hours, at the nominal test concentrations 125, 250, 500, 1000, and 2000 mg/L. The obtained EC10 and EC50 values were 300 mg/L and 640 mg/L, respectively.