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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1953
Report Date:
1953

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
Method: BASF test: 50 µl of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed up to 24 days after treatment. Findings were recoded daily.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): n-Vinylimidazol
no further data

Test animals / tissue source

Species:
rabbit
Strain:
not specified

Test system

Vehicle:
water
Controls:
other: untreated eyes of the same animals
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): ca. 0.05-0.1 mL
- Concentration (if solution): undiluted and 10, 5, 1 and 0.1% aqueous solution
Duration of treatment / exposure:
single application
Observation period (in vivo):
up to 24 days
Number of animals or in vitro replicates:
2 for undiluted test substance, 1 per aqeous solution (10, 5, 1 and 0.1%)

Results and discussion

In vivo

Results
Irritation parameter:
other: severe redness of the conjunctivae, secretion of pus, bleeding of the conjunctivae, ciliary injection and severe oedema
Basis:
animal: 2 animals
Reversibility:
other: Corneal cloudiness was still reported at the end of the observation period (d24).
Remarks on result:
probability of severe irritation
Irritant / corrosive response data:
The pure substance caused severe redness of the conjunctivae, secretion of pus, bleeding of the conjunctivae, ciliary injection and severe oedema. Cloudiness of the cornea was still reported at the end of the observation period (day 24).
The 10 and 5% solutions caused redness and swelling of the conjunctivae, which had disappeared 2 to 3 days after exposure.
The 1 and 0.1% solutions caused slight redness of the conjunctivae, which had disappeared 1 day after exposure.

Applicant's summary and conclusion

Interpretation of results:
corrosive