(4-chlorophenyl)hydrazine

Repeated dose toxicity: oral

Administrative data

Endpoint:

short-term repeated dose toxicity: oral

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Limited documentation

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Sex dependent toxicity of four chemicals.

Author:

Toth

Year:

1975

Bibliographic source:

Res. Comm. Chem. Path. Pharmacol. 10, 577-580; cited in WHO 2000, Phenylhydrazine. CICAD 19, Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, ISBN 92 4 153019 7; ISSN 1020-6167.

Reference 2

Reference Type:

publication

Title:

Sex dependent toxicity of four chemicals.

Author:

Toth

Year:

1975

Bibliographic source:

Res. Comm. Chem. Path. Pharmacol. 10, 577-580; cited in BUA (1995) Phenylhydrazine. Stuttgart, S. Hirzel, Wissenschaftliche Verlagsgesellschaft (Report No. 120; ISBN 3-7776-0691-X).

Reference 3

Reference Type:

secondary source

Title:

Concise International Assessment Document 19: Phenylhydrazine

Author:

WHO

Year:

2000

Bibliographic source:

Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, ISBN 92 4 153019 7; ISSN 1020-6167

Reference 4

Reference Type:

secondary source

Title:

Unnamed

Year:

1994

Materials and methods

Principles of method if other than guideline:

Mice were treated with the test compound orally ad libitum in drinking water. Survival rates, water consumption, body weight and histological changes were determined.

GLP compliance:

no

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): phenylhydrazine HCl (phenylhydrazine hydrochloride; PH)

Test animals

Species:

mouse

Strain:

Swiss

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: from a colony bred randomly in the laboratory of the authors.

ENVIRONMENTAL CONDITIONS
- not reported

Administration / exposure

Route of administration:

oral: drinking water

Vehicle:

water

Details on oral exposure:

no data

Analytical verification of doses or concentrations:

not specified

Details on analytical verification of doses or concentrations:

no data

Duration of treatment / exposure:

28 days

Frequency of treatment:

daily

Doses / concentrations

Remarks:

Doses / Concentrations:
0.001 %
Basis:
nominal in water

No. of animals per sex per dose:

4

Control animals:

yes

Details on study design:

no data

Positive control:

no data

Examinations

Observations and examinations performed and frequency:

CAGE SIDE OBSERVATIONS: yes
- Time schedule: at least every 4 days

BODY WEIGHT: Yes
- Time schedule for examinations: no data

WATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study): Yes
- Time schedule for examinations: no data

Sacrifice and pathology:

GROSS PATHOLOGY and HISTOPATHOLOGY: Yes

Other examinations:

no data

Statistics:

no data

Results and discussion

Results of examinations

Details on results:

CLINICAL SIGNS AND MORTALITY
Survival rates at four day intervals up to 28 days: see Table 1
Phenylhydrazine, administered daily in drinking water exert a stronger toxic effect in male mice compared to female mice. Whereas no female mice died up to 28 days, male mice died between 4 and 28 days of treatment. Only one male of 4 males survived.

BODY WEIGHT AND WEIGHT GAIN
Decreased substantially; the decrease in body weight was the most pronounced at one week after the application of chemicals followed by a gradual increase.

WATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study)
In the phenylhydrazine treated groups the animals consumed 30% less than in the corresponding untreated controls.

HISTOPATHOLOGY: NON-NEOPLASTIC
Pheylhydrazine caused congested lungs. In addition, slight fatty degeneration in the liver was observed.

Effect levels

Dose descriptor:

dose level:

Effect level:

0.001 other: %

Sex:

male/female

Basis for effect level:

other: mortality; body weight loss; reduced water consumption, congested lungs, slight fatty degeneration in the liver.

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

Table 1: Sex dependent toxicity of phenylhydrazine

Chemical	Dose	No	Sex	No of survivors (age in days)
				4	8	12	16	20	24	28
Phenylhydrazine	0.001% in drinking water	4	females	4	4	4	4	4	4	4
		4	males	3	3	2	2	2	2	1