[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Significant methodological deficiencies (unsuitable vapour/dust generating system: animals were not sufficiently exposed)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

(whole body exposure for 8 h, no analytics to determine concentrations or particle size distribution, 8 day observation period)

Principles of method if other than guideline:

BASF test (IHT):
The test demonstrates the toxicity of an atmosphere enriched with dust of the test substance at room temperature. Young adult laboratory rats, 6 per sex, were exposed sequentially to the dust generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 8 h. The nominal concentration was calculated as quotient of the amount of test substance weight loss during exposure, and the amount of air used during exposure. Group-wise documentation of clinical signs was performed over a 7 day study period. Body weight of groups was determined before the start of the study and at the end of the observation period.

GLP compliance:

no

Test type:

other: inhalation hazard test

Test material

Test material information

Constituent 1

Reference substance name:

[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

EC Number:

249-125-4

EC Name:

[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

Cas Number:

28654-73-1

Molecular formula:

C47 H49 Cu N11

IUPAC Name:

Copper,[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyanine-C,C,C-trimethanaminato(2-)-κN29,κN30,κN31,κN32]-

Details on test material:

Please refer to confidential details on test material

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation (mean values): males: 203 g, females: 178 g (group #1); males: 179 g, females: 193 g (group #2); males/females: 183 g (control group)

ENVIRONMENTAL CONDITIONS: not reported

Administration / exposure

Route of administration:

inhalation: dust

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: exsiccator
- Source and rate of air: 200 L/h
- Temperature in air chamber: 20°C
- System of generating particulates/aerosols: bubbling 200 L/h air through a substance column


TEST ATMOSPHERE
- Brief description of analytical method used: reweighing of a substance column
- Samples taken from breathing zone: no

VEHICLE
- Composition of vehicle: air
- Concentration of test material in vehicle: 0.68 mg/L (nominal conc.)

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

8 h

Concentrations:

0.68 mg/L (nominal)
(Nominal concentration was calculated as quotient of the amount of test substance weight loss during the exposure and the amount of air used during the exposure.)

No. of animals per sex per dose:

Test group: 6 rats
Control group: 3 rats

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 8 days
- Frequency of weighing: before application and before sacrifice (day 8)
- Necropsy of survivors performed: yes

Statistics:

not performed

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC0

Effect level:

0.68 mg/L air (nominal)

Based on:

test mat.

Exp. duration:

8 h

Mortality:

No mortality was observed.

Clinical signs:

other: The animals showed slight irritations of mucous membrane which were reversible within 3 days.

Body weight:

Body weight were considered unaffected by the treatment with the test item.
Mean weight before application:
Test group#1: males: 203 g, females 178 g; test group#2: males: 179 g, females: 193 g; control group: males/females: 183 g
Mean weight before sacrifice:
Test group#1: males: 233 g, females 192 g; test group#2: males: 200 g, females: 202 g; control group: males: 226 g, females: 192 g

Gross pathology:

No abnormalities were observed.