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Ecotoxicological information

Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 July 1999 - 16 July 1999
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
Specific details on test material used for the study:
- Name of test material (as cited in study report): 2-Propanol, 1-(bis(3-dimethylamino)propyl)amino)-
- Substance type: organic
- Physical state: clear pale yellow liquid
- Analytical purity: not advised
- Impurities (identity and concentrations): not reported
- Lot/batch No.: 184-3-0998
- Storage condition of test material: at room temperature, dry conditions
- Expiration date of the lot/batch: 19 March 2000
Analytical monitoring:
Details on sampling:
- Samples for test substance concentration verification were taken at 15 min., 2, 4, 24, 48, 72 and 96 h exposure
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test concentrations were prepared by direct addition of an appropriate aliquot of the test substance to the exposure vessel containing 20 litres of diluent water.
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):
- Evidence of undissolved material (e.g. precipitate, surface film, etc):
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
- Common name: rainbow trout
- Source: River Gwash Trout Farm Ltd, Lincolnshire, UK
- Age at study initiation (mean and range, SD): not reported (juvenile)
- Length at study initiation (length definition, mean, range and SD): 5.1 cm (SD=0.3 cm)
- Weight at study initiation (mean and range, SD): 2.29 g (SD=0.46 g)
- Feeding during test: no

- Acclimation period: approx 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: commercial fish food (amount not specified)
- Feeding frequency: daily
- Prophylactic treatment: no medication was given during the acclimatisation
- Health during acclimation (any mortality observed): no mortalities recorded within 7 days prior to the start of the study
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
135-159 mg/L expressed as CaCO3
Test temperature:
11-14 °C
Dissolved oxygen:
9.0-9.3 mg O2/L
Not applicable
Nominal and measured concentrations:
Nominal: 10, 22, 46, 100 and 220 mg/L
Details on test conditions:
- Test vessel: glas vessels
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 20 L, 25x46x25 cm
- Aeration: yes, gently
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

- Source/preparation of dilution water: as described under "details on test solution"
- Total organic carbon: 2.3 mg/L
- Particulate matter: <0.5 mg/L
- Metals: < 0.01 mg/L
- Pesticides: < 0.02 μg/L (organo chlorine), < 0.02 μg/L (organo phosphorus)
- Chlorine: < 0.05 μg/L, as residual chlorine
- Alkalinity: 98 mg/L as CaCO3
- Conductivity: 420 µS/cm
- Intervals of water quality measurement: pH and conductivity daily, alkalinity, hardness and free & total chlorine once per week, other parameters quarterly.
- During the test, pH, temperature, and dissolved oxygen concentrations were measured daily.

- Adjustment of pH: from 9.2 to 7.9 using 1M HCl
- Photoperiod: 16h day, 8h night
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality and incidence of sub-lethal effects (at 15 min, 2, 4, 24, 48, 72 and 96h)

- Test concentrations: 10, 22, 46, 100 and 220 mg/L
Key result
96 h
Dose descriptor:
Effect conc.:
148 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities: no
- Observations on body length and weight: not reported
- Other biological observations: marked reactions to exposure (oter than death) were increased pigmentation, darkened, swimming on the bottom, cloudy eyes, loss of equilibrium, hyperventilating, swimming at surface, resting on the bottom and lying on the bottom
- Mortality of control: no
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
Reported statistics and error estimates:
Validity criteria fulfilled:
Rainbow trout (Oncorhynchus mykiss) was exposed to the test substance at 10, 22, 46, 100 and 220 mg/L. The 96 hr LC50 is concluded to be 148 mg/L.

Description of key information

A test was performed under GLP conditions and according to OECD TG 203. An LC50 was found for Rainbow trout (Oncorhynchus mykiss) of 148 mg/L after an exposure of 96 hours in a semi static test (Huntsman, 1999).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
Effect concentration:
148 mg/L

Additional information