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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and based on the time-weighted mean measured test concentrations gave a 48-Hour EC50 value of 1.8 mg/L with 95% confidence limits of 1.7 - 2.0 mg/L. The study was performed according to GLP and OECD Guideline 202.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1.8 mg/L

Additional information

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at concentrations of 1.0,1.8, 3.2, 5.6 and 10 mg/L for 48 hours at a temperature of 20 to 21°C under semi-static test conditions. The number of immobilized Daphnia was recorded after 24 and 48 hours.

Chemical analysis of the freshly prepared test preparations at 0 and 24 hours showed measured test concentrations to range from 89% to 97% of nominal. A decline in measured test concentrations was observed in the old or expired test media at 24 and 48 hours in the range of 60% to 83% of nominal. Given this decline in measured test concentrations it was considered justifiable to base the results on the time-weighted mean measured test concentrations. The 48-Hour EC50 value based on the time-weighted mean measured test concentrations was 1.8 mg/L with 95% confidence limits of 1.7 -2.0 mg/L. The No Observed Effect Concentration was 0.77 mg/L.

The study met the acceptability criteria prescribed by the protocol and was considered valid.