1,3-diethyl-2-thiourea

Developmental toxicity / teratogenicity

Administrative data

Endpoint:

developmental toxicity

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Comparable to guideline study [OECD 414] A read-across between DMTU (dimethylthiourea) and DETU (diethylthiourea) is justified because theirs structures differ by a methyl fonction only. DETU has a molecular weight more higher than DMTU and therefore DETU is probably less toxic than DMTU.

Data source

Reference

Reference Type:

publication

Title:

Difference in the developmental toxicity of ethylenethiourea and three N,N-substituted thiourea derivatives in rats.

Author:

Saillenfait AM, Sabate JP, Langonne J and de Ceaurriz J.

Year:

1991

Bibliographic source:

Fundam. Appl. Toxicol. 17: 399-408

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 414 (Prenatal Developmental Toxicity Study)

Deviations:

no

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dimethyl-2-thiourea

EC Number:

208-588-2

EC Name:

1,3-dimethyl-2-thiourea

Cas Number:

534-13-4

Molecular formula:

C3H8N2S

Constituent 2

Reference substance name:

N,N'-Dimethylthiourea

IUPAC Name:

N,N'-Dimethylthiourea

Test animals

Species:

rat

Strain:

Sprague-Dawley

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: From IFFA CREDO Breeding Laboratories (St Germain sur l'arbresle, France)
- Age at study initiation: no data
- Weight at study initiation: males = 350 g ; females = 200-220 g
- Fasting period before study: no data
- Housing: Bred females were individually housed in clear polycrbonate cages with hardwood shavings as bedding.
- Diet (e.g. ad libitum): UAR Alimentation Villemoisson, ad libitum
- Water (e.g. ad libitum): filetred tap water, ad libitum
- Acclimation period: 1 or 2 weeks

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- °C
- Humidity (%): 55 +/- 5%
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): a light cycle from 7AM to 7 PM

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on exposure:

Administered volume = 5 ml/kg bw
The actual volume administered was based on body weight taken on GD6.

Analytical verification of doses or concentrations:

not specified

Details on mating procedure:

- Impregnation procedure: cohoused
- If cohoused:
- M/F ratio per cage: 1M / 3 F
- Length of cohabitation: overnight
- Verification of same strain and source of both sexes: yes
- Proof of pregnancy: sperm in vaginal smear referred to as day 0 of pregnancy

Duration of treatment / exposure:

gestation days 6 to 20

Frequency of treatment:

daily, at approximately the same time each time

Duration of test:

1 month

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 15, 25, 50, 100 and 200 mg/kg bw/day
Basis:
actual ingested

No. of animals per sex per dose:

20-23 animals/dose

Control animals:

yes, concurrent vehicle

Examinations

Maternal examinations:

CAGE SIDE OBSERVATIONS: Yes, daily
DETAILED CLINICAL: no data
BODY WEIGHT : Yes, on day 0 and every 3 days from days 6 to 21 of gestation.
POST-MORTEM EXAMINATIONS: Yes
- Sacrifice on gestation day 21
- Organs examined: uterus

Ovaries and uterine content:

The ovaries and uterine content was examined after termination: Yes
Examinations included:
- Gravid uterus weight: No data
- Number of corpora lutea: No data
- Number of implantations: Yes
- Number of early resorptions: Yes
- Number of late resorptions: Yes
- Live and dead fetuses

Fetal examinations:

Fetal body weight & Fetal malformations
live fetuses were removed, weighed, sexed and examined.
- External examinations: Yes: all per litter (including those of the oral cavity)
- Soft tissue examinations: Yes: half per litter
- Skeletal examinations: Yes:half per litter
- Head examinations: Yes: No data

Statistics:

Whenever possible, the data were presented as means ± SD. Implantation sites, live fetuses, and various body weights were analyzed by the one-way analysis of variance, followed by Dunnett's test if differences were found. The frequency of nonsurviving implants, resorptions, and anomalies among litters was evaluated with the Dixon-Massey test after an arc-sine-square root transformation. Rates of pregnancy and fetal sex ratio were analyzed using Fisher's exact test. Where applicable, least-squares analysis was carried out. The reported level of statistical significance was p < 0.05.

Indices:

no data

Historical control data:

no data

Results and discussion

Results: maternal animals

Maternal developmental toxicity

Details on maternal toxic effects:

No maternal deaths, morbidity, or treatment-related clinical signs were observed during this study. Significant reduction of maternal body weight gain was observed in tha last third of gestation at 15 mg/kg bw/d and throughout treatment at higher dose levels. The maternal weight gain during days 6 to 21 of gestation and the absolute weight gain were significantly reduced at all doses (p<0.01) (table 1). Incidences of pregnancy were comparable among groups (table 2).

Effect levels (maternal animals)

Dose descriptor:

NOAEL

Effect level:

< 15 mg/kg bw/day

Basis for effect level:

other: maternal toxicity

Results (fetuses)

Details on embryotoxic / teratogenic effects:

No significant effect of DMTU was noted on the mean numbers of implantation sites, viable fetuses, and percentage of resorptions and on fetal sex ratio. fetal body weights were significantly reduced at all doses tested, showing a dose-depenent relationship. The 9% decrease at 15 mg/kg bw/d reached 34% at 200 mg/kg bw/d (Table 2). The decrease in maternal weight was considered severe aand the fetal effects caused by DMTU were probably secondary to the toxicity incurred by the mothers.
No animalies were seen upon external examination of the fetuses (table 3). Dilated ureter and extra lumbar ribs occurred significantly more often among litters of rats given 200 mg/kg bw/d (p<0.01 and p<0.05, respectively). No other visceral and skeletal variants were significantly affected at any tested dose. In the control group, one fetus exhibited a forelimb syndactiyly and onother a bilateral microphtalmia.

Effect levels (fetuses)

1

Dose descriptor:

NOAEL

Effect level:

< 15 mg/kg bw/day

Sex:

male/female

Basis for effect level:

fetal/pup body weight changes

2

Dose descriptor:

NOAEL

Effect level:

> 200 mg/kg bw/day

Basis for effect level:

other: teratogenicity

Fetal abnormalities

Abnormalities:

not specified

Overall developmental toxicity

Developmental effects observed:

not specified

Any other information on results incl. tables

Table 1 : change in weight during gestation in SD rats treated daily by gastric intubation with DMTU on days 6 to 20 of gestation and sacrified on day 21^a

Compound	Dose	Body weight (g) on GD6	Body weight gain (g)						Absolute weight gain (g)^b
			GD 6-9	GD 9-12	GD 12/15	GD 15-18	GD 18-21	GD 6-21
Distilled water (ml/kg/d)	5	257+/-14	18+/-5	16+/-6	24+/-5	48+/-7	59+/-10	162+/-19	46+/-17
DMTU (mg/kg/d)	15	255+/-16	15+/-4	15+/-5	19+/-6	40+/-9*	46+/-11**	135+/-24**	33+/-10**
	25	255+/-14	12+/-6*	16+/-5	17+/-6**	38+/-9**	41+/-10**	126+/-24**	28+/-13**
	50	256+/-11	6+/-5**	19+/-6	15+/-5**	35+/-11**	34+/-9**	109+/-21**	19+/-10**
	100	253+/-15	3+/-10**	11+/-7*	14+/-7**	25+/-8**	35+/-9**	88+/-20**	3+/-10**
	200	257+/-16	-1+/-4**	14+/-7	8+/-6**	22+/-8**	27+/-9**	71+/-14**	-2+/-11**
Data are expressed as means +/- SD ^aIncludes all dams pregnant at sacrifice. GD = gestational day. ^b(day 21 bw) – (gravid uterus weight) – (day 6 bw) * and ** denote significant differences from the vehicle control value using Dunnett’s test, p<0.05 and p<0.01, respectively.

 

 

Table 2 : Reproductive parameters in SD rats treated daily by gastric intubation with DMTU on days 6 to 20 of gestation and sacrified on day 21^a

Compound	Dose	No. of deaths per number of treated females	% of females pregnant	Number of examined litters	Mean implantation sites per litter	Mean live fetuses per litter	Mean % nonsurviving implants per litter^b	Mean % resorption sites per litter
Distilled water (ml/kg/d)	5	0/20	95.0	19	15.26+/-1.94	14.95+/-1.96	2.02+/-3.70	2.02+/-3.70
DMTU (mg/kg/d)	15	0/21	85.7	18	14.39+/-3.47	14.06+/-3.45	2.24+/-4.08	2.24+/-4.08
	25	0/21	95.2	20	14.50+/-3.32	14.15+/-3.59	3.63+/-6.44	3.63+/-6.44
	50	0/21	90.5	19	13.79+/-3.85	13.26+/-3.75	3.49+/-5.55	3.49+/-5.55
	100	0/23	95.6	22	14.81+/-3.17	14.04+/-2.90	4.73+/-6.14	4.73+/-6.14
	200	0/23	87.0	20	14.40+/-2.85	13.70+/-2.87	4.68+/-7.19	4.27+/-7.20
Data are expressed as means +/- SD ^aIncludes all dams pregnant at sacrifice. GD = gestational day. ^bResorption plus dead fetuses * and ** denote significant differences from the vehicle control value using Dunnett’s test, p<0.05 and p<0.01, respectively.

 

Compound	Dose	Fetal sex ratio M:F (%)	Mean fetal body weight (g) per litter
			Males	Females
Distilled water (ml/kg/d)	5	0.86	6.00+/-0.30	5.68+/-0.29
DMTU (mg/kg/d)	15	1.14	5.47+/-0.33**	5.19+/-0.34**
	25	0.96	5.29+/-0.30**	5.04+/-0.25**
	50	1.15	5.20+/-0.33**	4.80+/-0.36**
	100	0.98	4.40+/-0.28**	4.26+/-0.29**
	200	0.89	3.94+/-0.28**	3.76+/-0.24**
Data are expressed as means +/- SD * and ** denote significant differences from the vehicle control value using Dunnett’s test, p<0.05 and p<0.01, respectively.

 

 

Table 3 : Incidence of anomalies in fetuses of SD rats teated daily by gastric intubation with DMTU on days 6 to 20 of gestation and sacrified on day 21

 

 

	Distilled Water (ml/kg/d)	DMTU (mg/kg/d)
	5	15	25	50	100	200
	Number of fetuses (litters) examined
External examination	284 (19)	253 (18)	283 (20)	252 (19)	309 (22)	274 (20)
Soft tissue examination	142 (19)	127 (18)	142 (20)	126 (19)	154 (22)	137 (20)
Skeletal examination	142 (19)	126 (18)	141 (20)	126 (19)	155 (22)	137 (20)
	Number of fetuses (litters) affected
Soft tissue anomalies
Microphtalmia^b	1 (1)	0	0	0	0	0
Hydroureter	0	0	0	0	0	3 (2)
Dilated renal pelvis	1 (1)	0	0	0	1 (1)	5 (2)
Dilated ureter	0	0	0	0	1 (1)	18 (11) **
Skeletal anomalies Interparietal and/or occipital not ossified	0	0	0	0	0	0
Vertebral centra dumbbell-shaped or absent	0	2 (2)	0	2 (2)	3 (3)	4 (3)
Extra lumbar ribs	9 (4)	9 (6)	8 (7)	13 (6)	14 (8)	13 (13)*
Fifth sternebrae Not ossified	4 (4)	0	1 (1)	0	0	2 (2)
Forelimb syndactilyly	1 (1)	0	0	0	0	0

 

^bConsidered to be a malformation * and ** denote significant differences from the vehicle control value, p<0.05 and p<0.01, respectively.

 

Applicant's summary and conclusion

Conclusions:

DMTU was not teratogenic in rat at 200 mg/kg bw/day, which was maternally toxic. But DMTU was fetotoxic at all doses tested in the presence of maternal toxicity.

Executive summary:

A read-across between these two substances is justified for the reproduction endpoint.

Because DETU and DMTU are two thioureas having a high structural similarity: both structures differ by a carbon by chain.

Moreover DETU and DMTU have the same physicalchemical properties, and are harmful by oral route (acute toxicity).

Saillenfait,et al (1991), tested DMTU in SD rats at 0, 15, 25, 50, 100 or 200 mg/kg/day from GD 6-20.

The only maternal toxicity noted was decreased body weight gain at 15 mg/kg/day and above (NOAEL of maternal toxicity < 15 mg/kg bw/d). The LOAEL for developmental toxicity was 15 mg/kg/day based on decrease of fetal body weight gain at all doses. The decrease in maternal weight was considered severe and the fetal effects caused by DMTU were probably secondary to the toxicity incurred by the mothers. Dilated ureter and extra lumbar ribs were observed at 200 mg/kg bw/d (significant results).

No teratogenic effects related to administration of DMTU were observed at dose levels up to 200 mg/kg bw/d, a level which was toxic to the dams.