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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

toxicity to soil macroorganisms except arthropods: short-term
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
no data available
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well documented study, without GLP

Data source

Reference Type:
the relative toxicity of metaldhyde and iron phosphate-based molluscicides to earthworms
Edwards, C.; Arancon, N.; Vasko-Bennett, M.; Little, B. & Askar, A.
Bibliographic source:
Crop Protection 28, 289-294

Materials and methods

Test guideline
according to guideline
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Edetic acid
EC Number:
EC Name:
Edetic acid
Cas Number:
2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid
Details on test material:
no details available
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no details available

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
no data

Test substrate

Details on preparation and application of test substrate:
EDTA were applied in deionized water solution into artificial soil (35 % dry weight moisture content).

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
Details on test organisms:
no data available

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
Total exposure duration:
14 d
Post exposure observation period:
no data available

Test conditions

Test temperature:
20 °C
no data available
35 % of maximum holding capacity
Details on test conditions:
Glass jars were used as test containers. 750 g of artificial soil which had been treated with one of the test chemicals (or a control with no chemicals) were placed in each jar. Ten E. fetida each with live weights of approximately 1 mg were added to each jar. There were four replicates of each chemical and dose.
Nominal and measured concentrations:
0, 0.1, 1.0, 10.0, 100.0, 1000.0 and 10000.0 mg/KG dry weight soil.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
14 d
Dose descriptor:
Effect conc.:
156.46 mg/kg soil dw
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: 136.01 - 179.98
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
Data were analyzed statistically by analysis of variance using S.A.S. software.
The LD50 values were estimated using Trimmed Spearman–Karber Version 1.5 (S.E.P.A. Cincinnati, Ohio) designed for analysis of mortality data from acute chronic toxicity tests.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156 mg/kg dry weight soil.
Executive summary:

The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156 mg/kg dry weight soil.