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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
acute, 48 hours, 28 to 30 June 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline and GLP-compliant study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): BEPD
- Physical state: colourless solid
- Analytical purity: 99.5%
- Lot/batch No.: HRC accession number 20.4.95/1
- Expiration date of the lot/batch: 31 December 1995.
- Storage condition of test material: in darkness at room temperature (ca. 20 degrees C).
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples were taken at 0 hours (fresh media) and 48 hours (expired media).

Test solutions

Vehicle:
no
Details on test solutions:
The test material was dispersed in test water with the aid of ultrasonic disruption for 30 minutes.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Test organism:
- Common name: Daphnia magna
- Source: laboratory culture
- Age at study initiation (mean and range, SD):

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
Not applicable

Test conditions

Test temperature:
21 - 22 °C
pH:
7.9 - 8.3
Dissolved oxygen:
7.6 - 8.3 mg/L
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal: 0 (control), 100 mg/L
Measured: 0 (control), 95 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Not applicable

Any other information on results incl. tables

The results of the definitive test are compiled below:

Nominal BEPD concentration (mg/L)

Mean BEPD concentration (mg/L)

Mean immobilisation after 48 hours (%)

0 hours

48 hours (E)

Mean

Mean as % of nominal

Control

ND

ND

-

-

0

100

96.5

93.44

95

95

0

ND: None detected

E: Expired media

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48 hour acute toxicity of BEPD toDaphnia magna wascarried out in a static system according to OECD 202 and EU guidelines. First instar daphnids were exposed over a 48 hour period to BEPD at nominal concentrations of 0 (control) and 100 mg/L. Duplicate, loosely covered vessels, each containing 10 daphnids in 200 ml medium were allocated to each treatment. Concentration of BEPD was verified analytically at test initiation and termination and mean measured concentration was 95 mg/L. Individual pH, temperature and dissolved oxygen values remained within acceptable limits throughout the duration of the study. The 48-hour EC50 was determined to be >100 mg BEPD/L (nominal).
Executive summary:

The 48 hour acute toxicity of BEPD to Daphnia magna was carried out in a static system according to OECD 202 and EU guidelines. First instar daphnids were exposed over a 48 hour period to BEPD at nominal concentrations of 0 (control) and 100 mg/L. Duplicate, loosely covered vessels, each containing 10 daphnids in 200 ml medium were allocated to each treatment. Concentration of BEPD was verified analytically at test initiation and termination and mean measured concentration was 95 mg/L. Individual pH, temperature and dissolved oxygen values remained within acceptable limits throughout the duration of the study. The 48-hour EC50 was determined to be >100 mg BEPD/L (nominal).