2-methoxyethyl 2-cyanoacrylate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study report data

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-methoxyethyl 2-cyanoacrylate

EC Number:

248-670-5

EC Name:

2-methoxyethyl 2-cyanoacrylate

Cas Number:

27816-23-5

Molecular formula:

C7H9NO3

IUPAC Name:

2-methoxyethyl 2-cyanoprop-2-enoate

Test material form:

liquid

Details on test material:

- Batch: P280004
- Appearance: clear, colourless liquid

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Test concentrations with justification for top dose:

50 to 5000 µg per plate

Vehicle / solvent:

acetone

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

acetone

True negative controls:

no

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

sodium azide

benzo(a)pyrene

other: 2-aminoanthracene

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Applicant's summary and conclusion

Conclusions:

The test material ß-methoxy-iso-propyl cyanoacrylate, was devoid of mutagenic activity under the conditions of test.

Executive summary:

The material ß-methoxy-iso-propyl cyanoacrylate (hereafter referred to as MPCA) was examined for mutagenic activity in four histidine-dependent auxotrophs of Salmonella typhimurium, strains TA 98, TA 100, TA 1535 and TA 1537, using pour-plate assays. The procedures used complied with OECD Guideline for Testing of Chemicals No. 471, EPA Toxic Substances Control Act and Annex V of the sixth amendment (79/831/EEC) to the European Community Directive 67/548/EEC. Each test, in each strain, was conducted on two separate occasions.

The studies, which were conducted in the absence and presence of an activating system derived from rat liver (S-9 mix), employed a range of levels of MPCA from 50 to 5000 µg per plate, selected following a preliminary toxicity test in strain TA 98. All tests included solvent (acetone) controls with and without S-9 mix.

No increases in reversion to prototrophy were obtained with any of the four bacterial strains at the MPCA levels tested, either in the presence or absence of S-9 mix.

Marked increases in the number of revertant colonies were induced by the known mutagens benzo[a]pyrene, 2 -nitrofluorene, 2 -aminoanthracene, 9 -aminoacridine and sodium azide when examined under similar conditions.

It was concluded that MPCA was devoid of mutagenic activity under the conditions of the test.