Registration Dossier
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EC number: 939-524-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- pH
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- Additional physico-chemical information
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- Nanomaterial agglomeration / aggregation
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Samples were taken from all treatments at 0 and 24h.
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 3g/L (based on the active component) is prepared by disolving 16.077g of test substance in 500 mL demineralised water.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Source: LISEC laboratory culture
- Age: less than 24 hours old at the start of the test
- Age of the parent daphnids: about three weeks old - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.3 - 21.3°C
- pH:
- 7.3 - 8.29
- Dissolved oxygen:
- >= 2.5 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations based on the active component: 0, 6.25, 12.5, 25, 50 and 100 mg/L
- Details on test conditions:
- - Renewal of the test solutions: after 24 hours
- Test containers: 50 mL borosilicate glass beakers
- Volume of test solution: 50 mL per vessel
- Number of organisms: 20 daphnids are used for the blank and for each test concentration divided into four groups of 5 animals
- Feeding: the daphnids are not fed during the test
- Because of the photodegradation of the test substance under light, the test was carried out entirely in the dark - Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: USEPA 1975 Methods for acute toxicity tests with fish, macroinvertebrates and ampphibianes EPA-660/3-75-009
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- other: Penaeus duorarum
- Details on test organisms:
- TEST ORGANISM
- Common name: Penaeus duorarum, pink shrimp
- Source: Big Lagoon, FL
- Age at study initiation (mean and range, SD): juvenile shrimp
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 20 - 30 mm
- Feeding during test: no
ACCLIMATION
- Acclimation period: 14
- Acclimation medium (same as test or not): the same as in the test, natural seawater
- Health during acclimation (any mortality observed): mortality was estimated to be < 3 % - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 22 +/- 1 °C
- pH:
- after 96 hours: 7.8
- Dissolved oxygen:
- after 96 hours: 2.8 mg/L (36 % of saturation)
- Salinity:
- 20 °/oo
- Nominal and measured concentrations:
- Test concentrations nominal: control and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass jars
- Size, fill volume: 19 L, 15 L
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- natural seawater
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1000 ppm
- Results used to determine the conditions for the definitive study: yes - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 747 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 747 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
Referenceopen allclose all
Description of key information
With high probability acutely not harmful to aquatic invertebrates.
EC50 (48h) > 100 mg/L (nominal) for Daphnia magna (OECD 202)
LC50 (96h) > 747 mg/L (nominal) for Penaeus duorarum (salt water) (EPA-660/3-75-009)
Key value for chemical safety assessment
Additional information
The acute toxicity on aquatic invertebrates was investigated for ‘zinc phthalocyanine sulfonate’ with the waterflea Daphnia magna, in a GLP guideline study according to OECD 202 (Procter & Gamble 1994). The test daphnia were exposed to the substance for 48 hours in a semi-static test system. At test termination an EC50 > 100 mg/L (nominal) was determined.
In a supporting stuy the toxicity of ‘zinc phtalocyanine sulfonate’ to aquatic invertebrates was investigated according to USEPA 1975 Methods for acute toxicity tests with fish, macroinvertebrates and amphibianes EPA-660/3-75-009 (Procter & Gamble 1978). In this study the pink shrimp Penaeus duorarum was exposed to the substance for 96 hours in a salt water limit test with a nominal concentration of 1000 mg/L (nominal) under static conditions. At test termination the LC50 > 747 mg/L (nominal) was determined.
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