Reaction product of [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]zinc, sulphuric acid and caustic soda

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Samples were taken from all treatments at 0 and 24h.

Vehicle:

no

Details on test solutions:

A stock solution of 3g/L (based on the active component) is prepared by disolving 16.077g of test substance in 500 mL demineralised water.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: LISEC laboratory culture
- Age: less than 24 hours old at the start of the test
- Age of the parent daphnids: about three weeks old

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20.3 - 21.3°C

pH:

7.3 - 8.29

Dissolved oxygen:

>= 2.5 mg/L

Nominal and measured concentrations:

Nominal test concentrations based on the active component: 0, 6.25, 12.5, 25, 50 and 100 mg/L

Details on test conditions:

- Renewal of the test solutions: after 24 hours
- Test containers: 50 mL borosilicate glass beakers
- Volume of test solution: 50 mL per vessel
- Number of organisms: 20 daphnids are used for the blank and for each test concentration divided into four groups of 5 animals
- Feeding: the daphnids are not fed during the test
- Because of the photodegradation of the test substance under light, the test was carried out entirely in the dark

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Qualifier:

according to guideline

Guideline:

other: USEPA 1975 Methods for acute toxicity tests with fish, macroinvertebrates and ampphibianes EPA-660/3-75-009

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: Penaeus duorarum

Details on test organisms:

TEST ORGANISM
- Common name: Penaeus duorarum, pink shrimp
- Source: Big Lagoon, FL
- Age at study initiation (mean and range, SD): juvenile shrimp
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 20 - 30 mm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14
- Acclimation medium (same as test or not): the same as in the test, natural seawater
- Health during acclimation (any mortality observed): mortality was estimated to be < 3 %

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

22 +/- 1 °C

pH:

after 96 hours: 7.8

Dissolved oxygen:

after 96 hours: 2.8 mg/L (36 % of saturation)

Salinity:

20 °/oo

Nominal and measured concentrations:

Test concentrations nominal: control and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass jars
- Size, fill volume: 19 L, 15 L
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- natural seawater

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1000 ppm
- Results used to determine the conditions for the definitive study: yes

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 747 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 747 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Description of key information

With high probability acutely not harmful to aquatic invertebrates.

EC50 (48h) > 100 mg/L (nominal) for Daphnia magna (OECD 202)

LC50 (96h) > 747 mg/L (nominal) for Penaeus duorarum (salt water) (EPA-660/3-75-009)

Additional information

The acute toxicity on aquatic invertebrates was investigated for ‘zinc phthalocyanine sulfonate’ with the waterflea Daphnia magna, in a GLP guideline study according to OECD 202 (Procter & Gamble 1994).  The test daphnia were exposed to the substance for 48 hours in a semi-static test system. At test termination an EC50 > 100 mg/L (nominal) was determined.

In a supporting stuy the toxicity of ‘zinc phtalocyanine sulfonate’ to aquatic invertebrates was investigated according to USEPA 1975 Methods for acute toxicity tests with fish, macroinvertebrates and amphibianes EPA-660/3-75-009 (Procter & Gamble 1978). In this study the pink shrimp Penaeus duorarum was exposed to the substance for 96 hours in a salt water limit test with a nominal concentration of 1000 mg/L (nominal) under static conditions. At test termination the LC50 > 747 mg/L (nominal) was determined.