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EC number: 222-182-2 | CAS number: 3380-34-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The publication contains sufficient detail to assume the validity of the study.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 427 (Skin Absorption: In Vivo Method)
- GLP compliance:
- no
Test material
- Reference substance name:
- Triclosan
- EC Number:
- 222-182-2
- EC Name:
- Triclosan
- Cas Number:
- 3380-34-5
- Molecular formula:
- C12H7Cl3O2
- IUPAC Name:
- 5-chloro-2-(2,4-dichlorophenoxy)phenol
- Details on test material:
- - Name of test material (as cited in study report): Labelled and unlabelled triclosan obtained from Environmental Safety Laboratory, Unilever Research, Sharnbrook, UK
- Physical state: no data
- Analytical purity: >99%
- Radiochemical purity (if radiolabelling): >99%
- Locations of the label (if radiolabelling): ³H-triclosan
Constituent 1
- Radiolabelling:
- yes
- Remarks:
- ³H-triclosan
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Comparative Biology Centre, The Medical School, University of Newcastle upon Tyne
- Age at study initiation: not specified
- Weight at study initiation: ca. 150 g
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: ethanol in water (9:1, v/v)
- Duration of exposure:
- 24 h
- Doses:
- About 0.2 mg/cm² of skin corresponding to a dose level of 1.9 mg/animal.
The specific radioactivity was 37.5 MBq/mg. - No. of animals per group:
- 3
- Control animals:
- yes
- Remarks:
- sham treatment
- Details on study design:
- - 24 hours prior to dosing the back of each animal was clipped free of hair. On the day of application, a silicone 'O' ring with an area of 9.6 cm² was attached to the back of each rat.
- The 3 females were dosed by application of 100 µL of 64.5 mM [³H]-triclosan in vehicle (concentration ca. 18.7 mg/mL) onto the skin area delimited by the silicone ring.
- Urine and feeces were collected from all animals at 1, 4, 7, and 24 h after dosing.
- At the 24 h sampling, rats were anaesthetised and heart blood was withdrawn. Animals were sacrificed and the dorsal application site was removed.
The skin surface at the application site was washed 6 times with a 3% Teepol solution and then wiped dry. Stratum corneum was removed form the application site by six tape strippings.
- radioactivity recovery was measured in urine, feeces, blood, organs, gauze wipes, carcass, skin, protective appliances and cages washings were
- Skin, urine and feces were worked up to allow analysis of parent compound, triclosan sulphate and triclosan glucuronide.
Results and discussion
- Signs and symptoms of toxicity:
- no effects
- Dermal irritation:
- no effects
- Absorption in different matrices:
- One hour after topical application of triclosan to rat skin in vivo, 75% of the dose remained on the skin surface. By 4-7 h, this had decreased and excretion in the urine increased. There was little lag time before the appearance of radiolabel in the carcass.
At 24 after dosing, 12% of radioactivity was in the feces, 8% in the carcass, 1% in the urine, 30% in the stratum corneum and 26% on the skin surface. Urinary radioactivity comprised free triclosan (50%), triclosan glucuronide (40%) and triclosan sulphate (10%) at 24 h.
Radioactivity was only detected in the 24-hr samples of faeces and comprised 5% of the dose as triclosan glucuronide and 3.5% of the dose as triclosan.
It was not possible to distinguish the contribution of dermal metabolism to the urine or faeces metabolite profile; however, triclosan glucuronide and triclosan sulphate as well as unchanged triclosan were extracted from skin at the site of application. 24 hr after dosing this contained 6.5% of the dose comprising triclosan (4%), triclosan glucuronide (1.5%) and triclosan sulphate. - Total recovery:
- Total recovery was 90.56±4.6% of dose after 24 hours. For further details see table below.
Percutaneous absorption
- Dose:
- 0.2 mg/cm²
- Parameter:
- percentage
- Absorption:
- ca. 21 %
- Remarks on result:
- other: 24 h
Any other information on results incl. tables
Distribution of radioactivity following dermal administration of ³H-TCS |
||
Sample |
% of dose ± SEM |
|
Urine |
0.88 ± 0.51 |
|
Faeces |
11.84 ± 2.5 |
|
Blood |
0.02 ± 0 |
|
Surface |
26.13 ± 0.7 |
|
Skin |
4.31 ± 1.34 |
|
Cage wash |
0.24 ± 0.2 |
|
Carcass digest |
7.72 ± 2.05 |
|
Stratum corneum |
36.33 ± 12.9 |
|
Skin cover |
1.38 ± 0.67 |
|
Recovery |
90.46 ± 4.6 |
|
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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