Triclosan

Short-term toxicity to fish

Description of key information

Acutely very toxic for aquatic organisms.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.54 mg/L

Additional information

The toxicity of Triclosan to fish was tested in a guideline study following EEC Directive, Annex V, Part. C.l. Zebra fish were exposed to the substance for 96 hours in a static system. At test termination a LC50 of 0.54 mg/l based on measured data was determined (Ciba-Geigy1990). A comparable result was obtained by a study set up according to U.S.-EPA guideline 66202/3-75-009. The study was conducted with Pimephales promelas as test organism in a static system. The LC50 after 96 hours was 0.26 mg/l based on nominal concentrations (Procter & Gamble 1990).

In a further study conducted with Lepomis macrochirus as test organism in a static system; the 96 -h LC50 was 0.37 µg/L (95% CL: 0.32 - 0.44 mg/L; nominal; Orvos et al., 2002). The study was performed according to US EPA national standards. Analytical monitoring was not performed.

The 96 -h LC50 was determined to be 4.37 mg/L for the transformation product methyltriclosan (CAS 4640 -01 -1). The study was performed without analytical monitoring, a guideline is not given, nevertheless, the basic data are given.

An overview on the available reliable data on the acute toxicity to fish is given in the following table.

Reference	RL	Species	Exp.	Effect level	Value (µg/L)	Nom./ meas.	Remark
Triclosan
Wang et al. (2013)	2	Carassius auratus	96 h	LC50	1839	nom	mortality
Oliveira et al. (2009)	2	Danio rerio	96 h	LC50	340	nom.	mortality
Ciba-Geigy Ltd. (1990). Test no.G 069 04	2	Danio rerio	96 h	LC50	540	meas.	mortality
Orvos et al. (2002)	2	Lepomis macrochirus	96 h	LC50	370	nom.	mortality
Wang et al. (2013)	2	Misgurnus anguillicaudatus	96 h	LC50	45	nom	mortality
Tamura et al. (2013)	2	Oryzias latipes	96 h	LC50	210	nom	mortality
Kim et al. (2009)	2	Oryzias latipes	96 h	LC50	600	nom.	mortality
Procter & Gamble (1990).Rep. No. 38655	2	Pimephales promelas	96 h	LC50	260	nom.	mortality
Wang et al. (2013)	2	Pseudorasbora parva	96 h	LC50	71	nom	mortality
Wang et al. (2013)	2	Tanichthys albonubes	96 h	LC50	889	nom	mortality
Liang et al. (2013)	2	Xiphophorus helleri	96 h	LC50	1480	meas.	mortality
Belanger et al. (2013) cited in Busquet et al. (2014)	2	Danio rerio	96 h	LC50	283	nom.	fish embryo acute toxicity
Busquet et al. (2014)	2	Danio rerio	96 h	LC50	300	nom.	fish embryo acute toxicity
Oliveira et al. (2009)	2	Danio rerio	96 h	LC50	420	nom.	fish embryo acute toxicity
Ishibashi et al. (2004)	2	Oryzias latipes	96 h	LC50	399	nom.	fish embryo acute toxicity
Fritsch et al. (2013)	2	Pimephales promelas	96 h	LC50	190	nom.	fish embryo acute toxicity
Methyltriclosan
Ciba-Geigy (1985). Report date: 13 Feb 1985	1	Danio rerio	96 h	EC50	4370	nom.	mortality

Exp.: exposure duration; Meas.: measured; Nom.: nominal; RL: Reliability