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Acute tests on all three trophic levels were performed to examine the aquatic toxicity of 2-methylbut-3-yn-2-ol. Marine Invertebrates were found to be the most sensitive species revealing an EC50 (96h) of 359 mg/L. Algae and aquatic invertebrates showed comparable sensitivity with an ErC50 (72h) > 500 mg/L and an EC50 (48h) > 500 mg/L. Effects of 2-methylbut-3-yn-2-ol towards fish were found to occur at even higher concentrations providing a LC50 (96h) of 3400 mg/L. Thus, 2-methylbut-3-yn-2-ol is considered to be acutely not harmful for aquatic organisms.