Registration Dossier
Registration Dossier
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EC number: 202-870-9 | CAS number: 100-61-8
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: 2e. Study well documented, meets generally accepted scientific principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Guideline:
- other: NEN 6501 (1980) and OECD 201 (older version)
- GLP compliance:
- no
Test material
- Reference substance name:
- Aniline
- EC Number:
- 200-539-3
- EC Name:
- Aniline
- Cas Number:
- 62-53-3
- Molecular formula:
- C6H7N
- IUPAC Name:
- aniline
- Reference substance name:
- 2-methylaniline
- IUPAC Name:
- 2-methylaniline
- Reference substance name:
- 3-methylaniline
- IUPAC Name:
- 3-methylaniline
- Reference substance name:
- 4-methylaniline
- IUPAC Name:
- 4-methylaniline
- Details on test material:
- Aniline: purity = pro analysis; LogPoct=1.03 (0.90); Mol weight= 93.13;Chs= 0.00
2-methylaniline: purity >= 99.5%; LogPoct=1.54; Mol weight= 107.16;Chs= 0.10
3-methylaniline: purity >= 99%; LogPoct=1.54; Mol weight= 107.16;Chs= - 0.07
4-methylaniline: purity > 97%; LogPoct=1.54; Mol weight= 107.16;Chs= -0.17
Chs: Hammett sigma constant, which is dependent on the position of the substituent.
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): isopropanol
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Age at study initiation (mean and range, SD): < 24 hr
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.08 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Aniline: 95% C.I.:0.06-0.10 mg/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 2-Methylaniline: 95% C.I.: 0.36-0.81 mg/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 3-Methylaniline
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 4-methylaniline: 95% C.I.:0.14-0.24 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- All anilines tested were acutely toxic to D. magna at concentrations below 1 mg/L. Compared to the methylated anilines, aniline was the most toxic.
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