Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 218-089-1 | CAS number: 2050-46-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December, 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to OECD guideline, but with nominal concentrations and without GLP compliance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- no
Test material
- Reference substance name:
- Ortho-Diethoxy Benzene
- IUPAC Name:
- Ortho-Diethoxy Benzene
- Reference substance name:
- 1,2-diethoxybenzene
- EC Number:
- 218-089-1
- EC Name:
- 1,2-diethoxybenzene
- Cas Number:
- 2050-46-6
- Molecular formula:
- C10H14O2
- IUPAC Name:
- 1,2-diethoxybenzene
- Reference substance name:
- ODEB
- IUPAC Name:
- ODEB
- Test material form:
- other: Crystallized solid mass at room temperature
- Details on test material:
- - Name of test material (as cited in study report): ODEB
- For more details, see below the "confidential details on test material"
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not relevant
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Due to the very low solubility in water, according to its Rhodia's SDS, the test item was submitted to a "water soluble fraction" approach, by placing it in four different flasks at concentrations of 1, 10, 100A and 100B mg/L.
- Method: Water soluble fraction
- Controls: no data
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no observed
Preparation of the test solution:
The day before the test, solutions of 100mg/L, 10mg/L and 1mg/L of the test substance were prepared in the test medium. The solutions were continuously stirred for 24 hours at approximately 25ºC. The solutions were brought to 20ºC before starting the test.
On the day of the test, the soluble fractions of each one of the four solutions were separated by decantation, during 2 hours. After that, they were introduced into the test bottles.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus, 1820 (Cladocera, Crustacea)
- Source: IAP (Instituto Ambiental do Parana)
- Age at study initiation (mean and range, SD): <24 hours
- Feeding during test : no
- Food type: unicellular green algae Scenedesmus subspicatus
- Amount: no data
- Frequency: once a day
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- 242 mg/L (as CaCO3)
- Test temperature:
- 19.3 - 20.4°C
- pH:
- 7.56 (mean of initial exposure measures) to 7.62 (mean of final exposure measures)
- Dissolved oxygen:
- 7.48 (initial) to 7.26 (final)
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal concentrations only : Control - 1 - 10 and 100 (duplicate) mg/L
- Reference substance (positive control):
- yes
- Remarks:
- Potassium Dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 - < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no data
- Mortality of control: no mortality or immobility observed
- Other adverse effects control: no observed
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: concentration nominal only - Results with reference substance (positive control):
- CE50 -24h Potassium Dichromate = 0.91 mg/L
95% confidence limits [0.86 - 0.97] mg/L
Any other information on results incl. tables
Initial Concentration (mg/L) |
Becher 1 (nb of mobile organisms) | Becher 2 (nb of mobile organisms) | ||
24h | 48h | 24h | 48h | |
100A | 5 | 2 | 4 | 1 |
100B | 7 | 1 | 5 | 1 |
10 | 10 | 10 | 10 | 10 |
1 | 10 | 10 | 10 | 10 |
Control | 10 | 10 | 10 | 10 |
Initial Concentration (mg/L) |
Immobile organisms 24h | Immobile organisms 48h | ||
Total number | Percentage | Total number | Percentage | |
100A | 11 | 55 | 17 | 85 |
100B | 8 | 40 | 18 | 90 |
10 | 0 | 0 | 0 | 0 |
1 | 0 | 0 | 0 | 0 |
Control | 0 | 0 | 0 | 0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Ortho-Diethoxy Benzene has an EC50 between 10 and 100 mg/L, according to study performed with OECD Guideline 202.
- Executive summary:
The 48 hours acute toxicity of Ortho-Diethoxy Benzenz to Daphnia magna was studied under static conditions. Daphnids were exposed to control and test chemical at nominal concentration from 1 to 100 mg/L (purity of 99.65%) for 48 hours (pH adjusted).The 48 hours EC50was between 10 and 100 mg/L.
Based on the results of this study, the test item was classified as harmful to Daphnia magna in accordance with the EC classification criteria.
Results Synopsis
Test Type: static
48h - EC50: between 10 and 100 mg/L
Endpoint(s) effected: mobility
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
