Dichloroacetyl chloride

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and OECD guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dichloroacetyl chloride

EC Number:

201-199-9

EC Name:

Dichloroacetyl chloride

Cas Number:

79-36-7

Molecular formula:

C2HCl3O

IUPAC Name:

2,2-dichloroacetyl chloride

Details on test material:

- Name of test material (as cited in study report):
- Lot/batch No.: 04 ILDC 005/6

- Physical state: liquid
- Analytical purity: 97%

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Hoechst AG
- Age at study initiation: 3-5 months
- Weight at study initiation: 2.2-2.8 kg
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20+/-3ºC
- Photoperiod (hrs dark / hrs light): 12 hours/12 hours

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

not specified

Controls:

no

Amount / concentration applied:

0.5 ml

Duration of treatment / exposure:

4 h or 3 min.

Observation period:

7 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: 2.5cm/2.5cm
- % coverage: form Beiersdorf comp.
- Type of wrap if used:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: 3min. or 4 hours

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

2

Max. score:

2

Reversibility:

not fully reversible within: 7 days

Remarks on result:

other: after 7 days open wound

Results 2

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

>= 1 - <= 2

Max. score:

2

Reversibility:

not fully reversible within: 7 days

Remarks on result:

other: after 7 days open wound

Results 3

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

3

Max. score:

3

Reversibility:

not reversible

Results 4

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

>= 2 - <= 3

Max. score:

3

Reversibility:

not fully reversible within: 72h

Irritant / corrosive response data:

corrosive

Other effects:

erithe and eschar. Edema.

Any other information on results incl. tables

Exposure 4 hours
time	30-60 min.	24h	48h	72h	7days
animal	1	1	1	1
Erytherm and eschar	2	2	2	2	impossible to judge – open wound
edema	2	2	1	1	impossible to judge – open wound
Skin discoloration	x	x	x	x
Erythema		x	x	x
Open wound, with redness					x

Exposure 3 minutes
time	30-60 min.	24h	48h	72h
animal	2	2	2	2
Erytherm and eschar	2	3	3	3
edema	2	2	3	2
Skin discoloration	x	x	x	x
Erythema	x	x	x	x
Open wound, with redness	x	x	x	x
Open wound				x

Applicant's summary and conclusion

Interpretation of results:

Category 1A (corrosive)

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The substancae applied caused severe burns of the animals' skin. The animal with exposure of 3 min. had erythema, edeme and wound 72 hours after exposition and the one with 4 hours of exposition had an open wound 7 days after the exposition.

Executive summary:

The substancae applied caused severe burns of the animals' skin. The animal with exposure of 3 min. had erythema, edeme and wound 72 hours after exposition and the one with 4 hours of exposition had an open wound 7 days after the exposition.