3-diethylaminophenol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non GLP guideline study, no chemical control analysis

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, part 9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-diethylaminophenol

EC Number:

202-090-9

EC Name:

3-diethylaminophenol

Cas Number:

91-68-9

Molecular formula:

C10H15NO

IUPAC Name:

3-(diethylamino)phenol

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: green algae (Scenedesmus subspicatus)
- Strain: DSM 96.81
-

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

23 +/-2 °C

Nominal and measured concentrations:

0, 0.63, 1.25, 2.5, 5, 10 mg/L nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glas tubes
- Type (delete if not applicable): open
- fill volume: 10 ml
- Aeration: no
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


GROWTH MEDIUM
- Standard medium used: yes (comparable to OECD 201)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: see OECD 201


OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: continuos illumination
- Light intensity and quality: 120 UE/m*m*s


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Other: Fluorescentce at 685 nm

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: 0, 0.63, 1.25, 2.5, 5, 10

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

7.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

5.08 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate