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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20/05/1991-01/06/1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Study has been conducted according to valid study guidelines. Deviations are in the number of fish per concentration and in the absent analytical confirmation of nominal concentrations. This is not expected to compromise the quality of the study results.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
Deviations are in the number of fish per concentration and in the absent analytical confirmation of nominal concentrations. This is not expected to compromise the quality of the study results.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
- Method of preparation: direct dispersion in water
- Media renewal: daily
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Parkwood Trout Farm, Kent, UK
- Length at study initiation: 4.1 ± 0.2 cm
- Weight at study initiation: 1.05 ± 0.13



ACCLIMATION
- Health during acclimation (any mortality observed): Mortality zero in the 7 days prior to the start of the test
- Medication: none


Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
24, 48 and 72 h
Hardness:
Approximately 50 mg/l as CaCO3
Test temperature:
14°C
pH:
not reported
Dissolved oxygen:
aeration: yes
Salinity:
fresh water study
Nominal and measured concentrations:
Range Finding Study: 1.0, 10 and 100 mg/l
Definitive Study: 1.0 mg/l
only nominal concentrations reported
Details on test conditions:
Medication: none
Test Vessels: 20 l
Loading: 0.53 g bodyweight/litre
Test water: dechlorinated tap water (sodium thiosulphate)
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Range Finding Study

Dates: 20 -24 may 1991

 Concentration

(mg/l)

          Cumulative Mortality (initial population = 3)

   24 Hours  48 Hours 72 Hours   96 Hours
 Control
1.0
10 2 2 2 2
 100 2 2

Definitive Study

Dates: 28 May - 1 June 1991

 Concentration

(mg/l)

          Cumulative Mortality (initial population = 10)

 

 24 Hours

 48 Hours

72 Hours 

 96 Hours

 Control

 1.0 R1

 1.0 R2

Validity criteria fulfilled:
yes
Conclusions:
The test substance is toxic to fish upon short-term exposure.
Executive summary:

A study was perform to assess the acute aquatic toxicity of the test material phenol 2 -(1-methylpropyl). The method followed the OECD Guidelines for Testing of Chemicals No. 203 "Fish, Acute Toxicity Test. Deviations are in the number of fish per concentration and in the absent analytical confirmation of nominal concentrations (this is not expected to compromise the quality of the study results). The screening 96 h acute toxicity study was conducted with rainbow trout at nominal test concentrations of 1.0, 10 and 100 mg/l. The number of fish was 3 per test concentration in a preliminary study. In the final study, the number of fish was 10 in two replicates at 1.0 mg/l. In parallel a control group were tested. Mortality ocurred (2) in the range finding study at 10 and 100 mg/l concentration. The LC50 at 96 hours exposure was determined to be >1 mg/l. The test substance is toxic to fish upon short-term exposure.

Description of key information

 LC50 at 96 hours >1 mg/L (OECD 203)

Key value for chemical safety assessment

LC50 for freshwater fish:
1 mg/L

Additional information

Phenol 2 -(1-methylpropyl) was evaluated for its aquatic toxicity. The LC50 at 96 hours exposure was determined to be >1 mg/l. The test substance is toxic to fish upon short-term exposure.

Test method:

The method followed the OECD Guidelines for Testing of Chemicals No. 203 "Fish, Acute Toxicity Test. Deviations are in the number of fish per concentration and in the absent analytical confirmation of nominal concentrations (this is not expected to compromise the quality of the study results). The screening 96 h acute toxicity study was conducted with rainbow trout at nominal test concentrations of 1.0, 10 and 100 mg/l. The number of fish was 3 per test concentration in a preliminary study. In the final study, the number of fish was 10 in two replicates at 1.0 mg/l. In parallel a control group were tested. Mortality ocurred (2) in the range finding study at 10 and 100 mg/l concentration.