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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009-2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DS/EN ISO 14669
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other aquatic crustacea: Acartia tonsa
Details on test organisms:
TEST ORGANISM
- Common name: Calanoid copepod (marine)
- Strain: Kept in culture at DHI since 1987, originally collected in the North Sea by Danmarks Fiskeriundersøgelser
- Source: DHI (cultured since 1987, strain originated in the North Sea)
- Age at study initiation : Large copepodites and adults

Study design

Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20.0 ± 0.3 ºC
pH:
7.8
Dissolved oxygen:
≥ 4 mg/l at test end
Salinity:
31.8 ‰
Nominal and measured concentrations:
Nominal concentrations: 0(control), 5, 10, 20, 50, 100 and 200 mg/l.
Because the substance breaks down very rapidly in contact with water, one of the breakdown products, IBP (CAS no.611-70-1 ), was measured instead to verify concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessels: Glass beakers with approx. 25 ml of test soulution

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
-

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (light:dark)
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
67 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Mortality: yes
- EC50/LC50: 0.58 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50(48 h) of Ethanamine, 2-ethoxy-N-(2-methyl-1-phenylpropylidene)-, CAS No.139007-06-0, is 67 mg/L for Acartia tonsa