Ethanamine, 2-ethoxy-N-(2-methyl-1-phenylpropylidene)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009-2010

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DS/EN ISO 14669

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(E)-2-Ethoxy-N-(2-methyl-1-phenylpropylidene)ethylamine

EC Number:

604-106-3

Cas Number:

139007-06-0

Molecular formula:

C14H21NO

IUPAC Name:

(E)-2-Ethoxy-N-(2-methyl-1-phenylpropylidene)ethylamine

Test material form:

other: liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other aquatic crustacea: Acartia tonsa

Details on test organisms:

TEST ORGANISM
- Common name: Calanoid copepod (marine)
- Strain: Kept in culture at DHI since 1987, originally collected in the North Sea by Danmarks Fiskeriundersøgelser
- Source: DHI (cultured since 1987, strain originated in the North Sea)
- Age at study initiation : Large copepodites and adults

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.0 ± 0.3 ºC

pH:

7.8

Dissolved oxygen:

≥ 4 mg/l at test end

Salinity:

31.8 ‰

Nominal and measured concentrations:

Nominal concentrations: 0(control), 5, 10, 20, 50, 100 and 200 mg/l.
Because the substance breaks down very rapidly in contact with water, one of the breakdown products, IBP (CAS no.611-70-1 ), was measured instead to verify concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessels: Glass beakers with approx. 25 ml of test soulution

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
-

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (light:dark)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

67 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: yes
- EC50/LC50: 0.58 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50(48 h) of Ethanamine, 2-ethoxy-N-(2-methyl-1-phenylpropylidene)-, CAS No.139007-06-0, is 67 mg/L for Acartia tonsa