Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009-2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: OSPAR Commission 2006: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry (Part B was amended in 2006 (OSPAR summary record 06/23/1, §9.7))
Deviations:
yes
Remarks:
Slightly smaller fish was used(3.55 ± 0.26 cm) than recomended in guideline (4-6 cm). This is considered to be of minor importance.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
Modified according to OSPAR Commission 2006: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations:(nominial) 0, 25, 50, 100 and 200 mg/l
- Sampling method: 40 ml taken out in 50 ml bottles of all testkoncentrations
- Sample storage conditions before analysis: Frozen at - 20 C right after sampling

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Scophtalmus maximus
Details on test organisms:
TEST ORGANISM
- Common name: Turbot
- Source: Maximus in Thisted, Denmark
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): 3.55 ± 0.26 cm
- Weight at study initiation (mean and range, SD): 1.06 ± 0.22 g

Study design

Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
16.9 ± 0.5 ºC
pH:
7.9
Dissolved oxygen:
>60 %
Salinity:
32 ‰
Nominal and measured concentrations:
Nominal concentrations: 0(control), 2, 5, 10, 25, 50, 100 and 200 mg/l.
Because the substance breaks down very rapidly in contact with water, one of the breakdown products, IBP (CAS no.611-70-1 ), was measured instead to verify concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessels: 20 l aquariums

- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: approx 1 g per l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
-

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (light:dark)


Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
61 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Mortality: yes
- LC50: 0.6 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50(96 h) of Ethanamine, 2-ethoxy-N-(2-methyl-1-phenylpropylidene)-, CAS No.139007-06-0, is 61 mg/L for Scophtalmus maximus