1,2-diphenoxyethane

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 20, 1984 to March 6, 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study carried out prior to GLP, but meets all other requirements

Justification for data waiving:

other:

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1,2-bis(phenoxy)ethane

IUPAC Name:

1,2-bis(phenoxy)ethane

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): 1,2-bis(phenoxy)ethane
- Molecular weight (if other than submission substance): 214.25
- Physical state: Colourless crystal at room temperature
- Analytical purity: 99% or more
- Other:
- Melting point: 96°C
- Solubility: Oil-soluble
- Solubility: 5% or more *
* DMSO

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

Concentration (µg/plate): 10, 50, 100, 500, 1000, 5000 with and without S9

Microbial suspension used in test: 0.1 ml
Test substance solutions: 0.1 ml
Sodium phosphate buffer: 0.5 ml
S9 Mix (With metabolic activation): 0.5 ml
Soft agar solution: 2.0 ml

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

2-acetylaminofluorene

9-aminoacridine

2-nitrofluorene

sodium azide

Details on test system and experimental conditions:

Source of strains used in test: The Institute of Environmental Toxicology
Date of receipt: 5 August 1979
Storage conditions (-80°C):
- Microbial suspension composition: 0.8 ml
- DMSO composition: 0.07 mL

S9 – Self manufactured on 15 February 1984. For details and composition of S9 mix see attached report

Test method: plating method

Test conditions:
Microbial suspension used in test: 0.1 ml
Test substance solutions: 0.1 ml
Sodium phosphate buffer: 0.5 ml
S9 Mix (With metabolic activation): 0.5 ml
Soft agar solution: 2.0 ml

Incubation Temperature: 3.7 °C
Time: 48 hours

Evaluation criteria:

See attached report

Statistics:

see attahced report

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

See attached report for results

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Test substance had no mutagenicity in all strains under the conditions of the study.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Test substance had no mutagenicity in all strains under the conditions of the study, in the absence or presence of metabolic activation.