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Sodium 2,4-dichlorophenoxyacetate was generally not acutely toxic to aquatic organisms. Daphnia magna showed the highest sensitivity to this substance after acute exposure (EC50 (48h) = 489.4 mg a.i./L) whereas no effects were observed in the remaining short-term studies performed with fish and algae up to the highest concentrations tested. The evaluation of the chronic toxicity was conducted using data from analogue substances.Lemna gibba showed the highest sensitivity after exposure to dimethylammonium 2,4-dichlorophenoxyacetate (EC50 = 0.38 mg a.i./L; NOEC = 0.18 mg a.i./L) after 14 days. Chronic effects were reported in aquatic invertebrates as well caused by exposure to the same substance. The results from the studies performed with the analogue substance 2,4-dichlorophenoxyacetic acid showed reduced larval fish survival after chronic exposure. No adverse effects of this substance on aquatic microorganisms could be observed.