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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline und GLP Study. Some minor deficiencies in documentation, especially on test substance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
Instead of 3,5-Dichlorophenol Copper-II-sulfate-pentahydrate was used as reference substance.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
5-Chlor-2-aminobenzotrifluorid D
IUPAC Name:
5-Chlor-2-aminobenzotrifluorid D
Constituent 2
Reference substance name:
HHKE 405
IUPAC Name:
HHKE 405

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Laboratory culture: Activated sludge of the domestic sewage treatement plant of city of Hildesheim
- Pretreatment: washing with dechlorinated and sterilized tap water
- Initial biomass concentration: 3.15 g/l

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
No data

Test conditions

Hardness:
no data
Test temperature:
18-22°C
pH:
controlled but not documented in report
Dissolved oxygen:
controlled but not documented in report
Salinity:
no data
Nominal and measured concentrations:
18, 32, 58, 100, 180, 320, 580, 1000 mg/l
Reference substance (positive control):
yes
Remarks:
Copper(II)sulfate pentahydrate

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
28 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
72 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
190 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 26 - < 204 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 95 % CL

Applicant's summary and conclusion

Validity criteria fulfilled:
yes