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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP, non-guideline, published , adequate for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1995

Materials and methods

Principles of method if other than guideline:
Method as described by Galloway S et al (1987) Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: evaluations of 108 chemicals Environ Mol. Mutagen, Vol. 10, pp. 1-175.
GLP compliance:
yes
Type of assay:
in vitro mammalian chromosome aberration test

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity > 99%.

Method

Species / strain
Species / strain:
other: Chinese hamster ovary (CHO) cells
Test concentrations with justification for top dose:
1600, 3000, 5000 µg/mL

Results and discussion

Test results
Genotoxicity:
negative
Remarks on result:
other:
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

No increases in chromosomal aberrations were noted in cultured CHO cells treated with isoprene, with or without S9.

Applicant's summary and conclusion

Conclusions:
Isoprene did not induce chromosomal aberrations in vitro in
cultures of Chinese hamster ovary cells.