Oxalonitrile

Repeated dose toxicity: dermal

Administrative data

Endpoint:

short-term repeated dose toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1960

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Materials and methods

Four male albino rabbits whose weights averaged 4.5 pounds were closely clipped of their fur, restrained in a wooden stock, and placed in the chamber. An especially designed door, through which the rabbits heads extended into glass jarsmaintained under positive air pressure, prevented the rabbits from breathing cyanogen while their bodies were being exposed and also made observation of the heads of the rabbits possible. The bodies of the rabbits were exposed to ethanedinitrile concentration of 10,000 ppm for a period of 8 hours.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Oxalonitrile

EC Number:

207-306-5

EC Name:

Oxalonitrile

Cas Number:

460-19-5

Molecular formula:

C2N2

IUPAC Name:

carbononitridic cyanide

Test material form:

gas

Specific details on test material used for the study:

99.5%

Test animals

Species:

rabbit

Sex:

male

Details on test animals or test system and environmental conditions:

Four male albino rabbits whose weights averaged 4.5 pounds were closely clipped of their fur, restrained in a wooden stock, and placed in the chamber. An especially designed door, through which the rabbits heads extended into glass jarsmaintained under positive air pressure, prevented the rabbits from breathing cyanogen while their bodies were being exposed and also made observation of the heads of the rabbits possible. The bodies of the rabbits were exposed to ethanedinitrile concentration of 10,000 ppm for a period of 8 hours.

Administration / exposure

Type of coverage:

open

Vehicle:

other: air

Details on exposure:

10,000 ppm (ca 21 g/m3) for 8 hours

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

8 hours

Doses / concentrations

Dose / conc.:

10 000 other: ppm

Remarks:

ca 21 g/m3

No. of animals per sex per dose:

4

Details on study design:

Four male albino rabbits whose weights averaged 4.5 pounds were closely clipped of their fur, restrained in a wooden stock, and placed in the chamber. An especially designed door, through which the rabbits heads extended into glass jarsmaintained under positive air pressure, prevented the rabbits from breathing cyanogen while their bodies were being exposed and also made observation of the heads of the rabbits possible. The bodies of the rabbits were exposed to ethanedinitrile concentration of 10,000 ppm for a period of 8 hours.

Results and discussion

Results of examinations

Clinical signs:

no effects observed

Dermal irritation:

no effects observed

Mortality:

no mortality observed

Effect levels

Key result

Dose descriptor:

NOEL

Effect level:

21 mg/cm² per day (nominal)

Basis for effect level:

clinical signs

dermal irritation

Target system / organ toxicity

Key result

Critical effects observed:

no

Applicant's summary and conclusion

Conclusions:

Dermal exposure of rabbits to ethanedinitrile gas in an acute toxicity study indicates that there is no intake of ethanedinitrile through skin. Dermal espousure of rabbits to 10,000 ppm (~21 g/m3) of ethanedinitrile for eight hours did not result in toxicity towards rabbits, or any clinical observations or macroscopic effects.

Executive summary:

Materials and methods

Four male albino rabbits whose weights averaged 4.5 pounds were closely clipped of their fur, restrained in a wooden stock, and placed in the chamber. An especially designed door, through which the rabbits heads extended into glass jarsmaintained under positive air pressure, prevented the rabbits from breathing cyanogen while their bodies were being exposed and also made observation of the heads of the rabbits possible. The bodies of the rabbits were exposed to ethanedinitrile concentration of 10,000 ppm for a period of 8 hours.

Summary and conclusion

Dermal exposure of rabbits to ethanedinitrile gas in an acute toxicity study indicates that there is no intake of ethanedinitrile through skin. Dermal espousure of rabbits to 10,000 ppm (~21 g/m3) of ethanedinitrile for eight hours did not result in toxicity towards rabbits, or any clinical observations or macroscopic effects.