Registration Dossier
Registration Dossier
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EC number: 939-460-0 | CAS number: 1471311-26-8
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The 48-Hour EL*50for the test item toDaphnia magnabased on nominal loading rates was 75 mg/L with 95% confidence limits of 56 - 100 mg/L loading rate WAF. The Lowest Observed Effect Loading rate was considered to be 100 mg/L loading rate WAF. The No Observed Effect Loading rate was 56 mg/L loading rate WAF.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 75 mg/L
Additional information
The key study was performed to US EPA Draft Ecological Effects Test Guidelines OPPTS 850.1010 and the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphniasp, Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
Following a preliminary range-finding test and an initial experiment, twenty daphnids (4 replicates of 5 animals) were exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L for 48 hours at a temperature of 21°C to 22 ºC under static test conditions. The number of immobilizedDaphniaand any adverse reactions to exposure were recorded after 24 and 48 hours.
The 48-Hour EL*50for the test item toDaphnia magnabased on nominal loading rates was 75 mg/L with 95% confidence limits of 56 - 100 mg/L loading rate WAF. The Lowest Observed Effect Loading rate was considered to be 100 mg/L loading rate WAF. The No Observed Effect Loading rate was 56 mg/L loading rate WAF.
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