Reaction product of propylidynetrimethanol, propoxylated, reaction products with ammonia and 2,2-Dimethyl-3-(4-morpholinyl)propanal

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2013-07-23 to 2013-07-25

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

31 May 2008

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13th April 2004

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

April 1996

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

For determination of the test item concentration, samples were taken from the testing concentrations and the control at the start and at the end of the test.

Vehicle:

no

Details on test solutions:

- Reconstituted water: ISO medium (according to OECD 202 guideline) was used as dilution water in the experiment.
Separate stock solutions of individual substances were first prepared in deionised water (prepared in the laboratory by MILLIPORE ELIX 3 water purification system). The ISO medium was prepared by adding 25 mL from each of four stock solutions to one litre deionised water.

Test solutions:
A stock solution of 100 mg/L (nominal) was first prepared by dissolving an amount of 0.05 g test item in 500 mL ISO medium using an overnight shaking (to obtain clear solution). The test solutions of the chosen test concentrations were prepared by appropriate diluting of this stock solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Species: Daphnia magna (Straus)
- Age at test start: Less than 24 hours old
- Sex: Female
- Origin: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection) 2100 Gödöllő, Kotlán S. u. 3. Hungary
- Breeding conditions: Daphnia were bred in the Laboratory of TOXI-COOP ZRT. under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test. The test animals were fed with centrifuged green alga suspension.

ACCLIMATION
Test animals were bred under similar conditions as that used during the exposure period, therefore additional acclimatisation before the test was not necessary.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

249 mg/L (as CaCO3)

Test temperature:

20.4 – 20.8 °C (test vessels); 20.2 – 21.4 °C (ambient temperature within the climate chamber)

pH:

7.18 – 8.11

Dissolved oxygen:

The dissolved oxygen concentration was in the range of 7.70 – 8.12 mg/L during the test.

Salinity:

not applicable

Nominal and measured concentrations:

Nominal concentrations: 6.3, 12.5, 25.0, 50.0 and 100.0 mg/L.
Measured concentrations (% of nominal) at the start of the test: 103, 95, 97, 94, 94
Measured concentrations (% of nominal) at the end of the test: 116, 112, 109, 108, 104
The analytically measured concentrations of the test item were within ± 20 % of the nominal during the experiment therefore the biological results were based on the nominal concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers of approximately 40 mL test medium
- Number of animals: Twenty Daphnia per test group, divided into four groups of five animals, each group in ~40 mL test medium.
- Aeration: no

Pre-Experiments:
In order to select appropriate test concentrations for use in the definitive test, a preliminary range-finding test was conducted to determine the approximate toxicity of the test item.
A stock solution of 100 mg/L (nominal concentration) was first prepared by dissolving the test item in ISO medium using an overnight shaking (to obtain clear solution). The test solutions were prepared by appropriate dilution of this stock solution. Ten daphnids (divided into two replicates) in each test concentration and control were exposed for 48 hours.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: The light-dark cycle during the test was 16 hours light (artificial illumination) and 8 hours darkness.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: test concentrations: 0; 0.1; 1; 10; 100 mg/L
- Results used to determine the conditions for the definitive study: immobilized animals: 1; 0; 0; 2; 10, respectively

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

45.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Biological results:
The test item concentrations remained within ± 20 % of the nominal during the test period of 48 hours, therefore all biological results are based on the nominal concentrations.
- Results after 24 hours:
The immobilization was 100 per cent at the highest tested concentration (100 mg/L) after 24 hours of exposure. Not significant immobility was observed (up to 10 per cent) at the concentration range of 6.3 – 50.0 mg/L. In the control no immobility was noticed.
The 24-h EC50 was calculated to be 60.8 mg/L using Probit analysis by SPSS PC+ software.
- Results after 48 hours:
Not significant immobility was observed (up to 10 per cent) at the concentrations of 6.3 and 12.5 mg/L. Accordingly, the 48-h NOEC was determined to be 12.5 mg/L. In the three highest test concentrations of 25.0, 50.0 and 100 mg/L significant immobility was noticed (20, 30, and 100% respectively). Accordingly, the 48-h LOEC was determined to be 25.0 mg/L and the 48-h EC100 was determined to be 100 mg/L.
The 48-h EC50 was calculated to be 45.1 mg/L using Probit analysis by SPSS PC+ software.

Results with reference substance (positive control):

The date of the last study with reference item Potassium dichromate was: 05 – 06 March 2013. The 24h EC50: 0.97 mg/L, (95 % confidence limits: 0.81 – 1.14 mg/L) .

The measured concentrations of the test item varied between 94 and 116 % of the nominal concentration during the experiment.

Validity criteria fulfilled:

yes

Conclusions:

In a 48-hour acute toxicity test with Daphnia magna the 48 h EC50 value was determined to be 45.1 mg/L.

Executive summary:

The 48-hour acute toxicity test with Daphnia magna was conducted in accordance with EU method C.2, OECD guideline no. 202 and EPA OPPTS 850.1010 guideline. The 48 h-EC50 value was determined as 45.1 mg/L, and the 48 h NOEC was determined to be 12.5 mg/L. The measured concentrations of the test item varied between 94 and 116 % of the nominal concentration during the experiment.Therefore, the results are based on the nominal test item concentrations.

Description of key information

In a 48-hour acute toxicity test with Daphnia magna the 48 h EC50 value was determined to be 45.1 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

45.1 mg/L

Additional information

The 48-hour acute toxicity test with Daphnia magna was conducted in accordance with EU method C.2, OECD guideline no. 202 and EPA OPPTS 850.1010 guideline. The 48 h-EC50 value was determined as 45.1 mg/L, and the 48 h NOEC was determined to be 12.5 mg/L. The measured concentrations of the test item varied between 94 and 116 % of the nominal concentration during the experiment.Therefore, the results are based on the nominal test item concentrations.