Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-01-15 to 1990-01-18
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: comparable to guideline study with acceptable restrictions (exposure concentrations in the test and the stability of the test substance were not confirmed by analysis)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412/9
Principles of method if other than guideline:
Method: other: Scenedesmus-Zellvermehrungshemmtest in Anlehnung an DIN 38412/9; Messung der Biomasseentwicklung
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N-methylformamide
EC Number:
204-624-6
EC Name:
N-methylformamide
Cas Number:
123-39-7
Molecular formula:
C2H5NO
IUPAC Name:
N-methylformamide
Details on test material:
- Name of test material (as cited in study report): Monomethyl formamide
- Other: Test substance as prescribed by 1.1 - 1.4

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
20 ±1 °C
pH:
8.1 - 8.8
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 250 mL erlenmeyer tube filled with 100 mL test solution
- Initial cells density: 10 000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


GROWTH MEDIUM
- Standard medium used: yes according to Din 38412 part 9


OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: permanent illumunation
- Light intensity and quality: 10000 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: EOS flourometer

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

Effect values (endpoint: biomass):

EbC10 (72 h) > 500 mg/L

EbC50 (72 h) > 500 mg/L

EbC90 (72 h) > 500 mg/L 

(values related to nominal concentrations)

Applicant's summary and conclusion