Registration Dossier
Registration Dossier
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EC number: 204-624-6 | CAS number: 123-39-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-01-15 to 1990-01-18
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: comparable to guideline study with acceptable restrictions (exposure concentrations in the test and the stability of the test substance were not confirmed by analysis)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412/9
- Principles of method if other than guideline:
- Method: other: Scenedesmus-Zellvermehrungshemmtest in Anlehnung an DIN 38412/9; Messung der Biomasseentwicklung
- GLP compliance:
- no
Test material
- Reference substance name:
- N-methylformamide
- EC Number:
- 204-624-6
- EC Name:
- N-methylformamide
- Cas Number:
- 123-39-7
- Molecular formula:
- C2H5NO
- IUPAC Name:
- N-methylformamide
- Details on test material:
- - Name of test material (as cited in study report): Monomethyl formamide
- Other: Test substance as prescribed by 1.1 - 1.4
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 20 ±1 °C
- pH:
- 8.1 - 8.8
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 250 mL erlenmeyer tube filled with 100 mL test solution
- Initial cells density: 10 000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
GROWTH MEDIUM
- Standard medium used: yes according to Din 38412 part 9
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: permanent illumunation
- Light intensity and quality: 10000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: EOS flourometer
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Any other information on results incl. tables
Effect values (endpoint: biomass):
EbC10 (72 h) > 500 mg/L
EbC50 (72 h) > 500 mg/L
EbC90 (72 h) > 500 mg/L
(values related to nominal concentrations)
Applicant's summary and conclusion
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