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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
THE TOXICITY AND TOXIC MANIFESTATIONS OF 2,2-BIS-(p-CHLOROPHENYL)-1,1,1-TRICHLOROETHANE (DDT) AS INFLUENCED BY CHEMICAL CHANGES IN THE MOLECULE: A Contribution to The Relation Between Chemical Constitution and Toxicological Action
Author:
W. F. von OETTINGEN and N. E. SHARPLESS
Year:
1946
Bibliographic source:
J Pharmacol Exp Ther December 1946 88:400-413
Reference Type:
publication
Title:
Journal of Pharmacology and Experimental Therapeutics
Year:
1946
Bibliographic source:
JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams & Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10- Volume(issue)/page/year: 88,400,1946
Reference Type:
review article or handbook
Title:
RTECS Number : SI7700000
Author:
RTECS database
Year:
2012
Bibliographic source:
RTECS (Registry of Toxic Effects of Chemical Substances); JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams & Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10- Volume(issue)/page/year: 88,400,1946

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
The toxicity and toxic manifestations of phenetole(as influenced bt chemical changes in DDT) in mice.
GLP compliance:
not specified
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
Phenetole
EC Number:
203-139-7
EC Name:
Phenetole
Cas Number:
103-73-1
Molecular formula:
C8H10O
IUPAC Name:
ethoxybenzene
Test material form:
liquid
Details on test material:
- Name of test material (as cited in study report): Phenetole
- Molecular Formula: C8H10O
- Molecular Weight: 122.17 g/mol
- Substance type: Organic
- Physical state: Liquid

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source:Not available
- Age at study initiation:Not available
- Weight at study initiation:20-30 gm
- Fasting period before study:Not available
- Housing:individual cages
- Diet (e.g. ad libitum):Purina dog chow(ad libitum)
- Water (e.g. ad libitum):water(ad libitum)
- Acclimation period:Not available

ENVIRONMENTAL CONDITIONS
- Temperature (°C):Not available
- Humidity (%):Not available
- Air changes (per hr):Not available
- Photoperiod (hrs dark / hrs light):Not available

IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
olive oil
Details on oral exposure:
VEHICLE
- Concentration in vehicle:5,10 or 25 percent solutions in olive oil
- Amount of vehicle (if gavage):Not available
- Justification for choice of vehicle:Not available
- Lot/batch no. (if required):Not available
- Purity:Not available

MAXIMUM DOSE VOLUME APPLIED:Not applicable

DOSAGE PREPARATION (if unusual):5,10 or 25 percent solutions in olive oil

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose:Not available
Doses:
1200,1400,1600,1800,2000 & 2200 mg/kg
No. of animals per sex per dose:
1200mg/kg-10
1400mg/kg-10
1600mg/kg-10
1800mg/kg-10
2000mg/kg-10
2200mg/kg-10
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations and weighing:no data
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs-incidence of tremors,convulsions & fatalities noted
Statistics:
Not availble

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
2 200 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50 percent mortality observed
Mortality:
50 percent mortality observed at 2200 mg/kg,whereas 80 percent mortality was seen at dose 2000 mg/kg and 10 percent mortality was seen at 1600 & 1800 mg/kg respectively.
Clinical signs:
Shortly after the administration the animals became depressed and with larger doses they passed into deep necrosis in 2-3 hrs and majority of animals died in a comatose condtion within 1-2 days.
Body weight:
Not available
Gross pathology:
Not available
Other findings:
Not available

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The acute oral median lethal dose (LD50) of phenetole in mouse was found to be 2200 mg/kg of body weight as 50 percent mortality was observed at this dose.
Executive summary:

In the study the acute oral toxicity of test compound was determined in white mice by orally administering the test compound in olive oil at doses 1200, 1400,1600,1800,2000 & 2200 mg/kg and observed for clinical signs and mortality for 1 week. The acute oral median lethal dose (LD50) of test chemical in mouse was found to be 2200 mg/kg of body weight (50 percent mortality observed), 80 percent mortality was seen at dose 2000 mg/kg and 10 percent mortality was seen at 1600 & 1800 mg/kg respectively. Thus considering the CLP criteria for classification it can be assessed that the given test chemical is non toxic via oral route as it exceeds the criteria of 2000 mg/kg bw for the substance to be classified as toxic.