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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
A sample was taken from each replicate vessel filled with the spiked substrate and from one replicate of the control susbstrate. Cadmium was analysed in these samples to verify the test concentration and to evaluate the homogeneity of the spiked substrate.
Vehicle:
no
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
Eisenia fetida Batches: RE9204-13 and RE9204-15
Origin: Janssen Pharmaceutica 1992
Breeding and stock maintenance: at the VITO test facility, according to SOP TREGV001.
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Details on test conditions:
6 replicates for control substrate and 6 replicates for the test substrate
Number of organisms at the start: 10 worms per vessel.
Test vessels: glass, 1000 ml with lid, previously washed in a standard laboratory dishwasher. During the test the vessels were closed, preventing evaporation of water.
Test volumes: approx. 500 g substrate per vessel (500.6-505.2 g variation within the 12 vessels used in the test).
Substrate: artificial OECD soil (Batch 13/01/2012) was used.
Composition of OECD artificial soil:
peat: 10%
kaolin: 20%
White sand (0.05-0.2 mm particle size): 70%
water: 360 ml/kg
Adjust to pH 6 +-0.5 with CaCO3


Conditions: test vessels were placed at room temperature (variation during the test: 19.6-22.2°C) and light/dark 16/8 (not critical).
Reference substance (positive control):
no
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
2.1 other: g/kg
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
CdTe
Basis for effect:
growth
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
1 other: g/kg DW
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Cd
Basis for effect:
growth
Details on results:
The mean weight loss in the control and in 2.1 g CdTe/kg was not statistically different.
Reported statistics and error estimates:
Student t-test comparing the replicate values of the spiked substrate to the values in the control.

Concentrations of Cd (g/kg DW) in replicate vessels at the start of the test. All vessels were filled with the same stock of spiked substane. It was concluded that the Cd concentration was sufficiently high and comparable in all test vessels to make conclusions on the effect of cadmium on the survival of earthworms exposed to these soil concentrations.

 vessel g/kg DW 
control 0.98 
test vessel 7  1.12 
test vessel 9  1.01 
test vessel 10  0.79 
test vessel 11  0.72 
test vessel 12  0.97 
mean  0.93 
sd  0.15 
VC  15.89 
Validity criteria fulfilled:
yes
Conclusions:
Test done according standard protocol.
Executive summary:

Solid CdTe is not acute toxic to earthworm when mixed into soil substrate up to concentrations of 2.1 g/kg Cd Te or 1g/kg of cadmium.

Description of key information

An ecotoxicity study was performed using Eisenia fetida as test species. No acute effects were seen up to a concentration of 2.1 g CdTe/kg DW or 1 g Cd/kg DW.
As Cd2+ ion determines ecotoxicity, the PNEC deriviation will be based on the Cd 2+ ion as determined in the RA (ECB 2007).

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
2 100 mg/kg soil dw

Additional information

Solid CdTe is not acute toxic to earthworm when mixed into soil substrate up to concentrations of 2.1 g/kg Cd Te or 1 g Cd/kg DW. As Cd2+ ion determines ecotoxicity, the PNEC deriviation for terrestrial compartment will be based on the Cd 2+ ion as determined in the RA (ECB 2007).