Isopentyl p-methoxycinnamate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

no data

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Guideline:

other: no data

GLP compliance:

no

Analytical monitoring:

not specified

Details on test solutions:

no details

Test organisms (species):

Daphnia sp.

Details on test organisms:

no details

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Remarks on exposure duration:

no data

Dose descriptor:

EC0

Effect conc.:

1 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Dose descriptor:

EC100

Effect conc.:

31.6 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Dose descriptor:

EC50

Effect conc.:

5.6 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: EC50 = EC0/EC100

Validity criteria fulfilled:

not specified

Conclusions:

In the acute test with daphnia an EC0 of 1 mg/L and and EC100 of 31.6 mg/L was determined. The EC50 was determined by caclulating the geometric mean of the EC0 and EC100. The EC50 is 5.6 mg/L.
No details about the test design (duration, test system, etc.) are known.

Executive summary:

In the acute test with daphnia an EC0 of 1 mg/L and and EC100 of 31.6 mg/L was determined. The EC50 was determined by caclulating the geometric mean of the EC0 and EC100. The EC50 is 5.6 mg/L. No details about the test design (duration, test system, etc.) are known.

Description of key information

The test substance is acutely toxic to aquatic invertebrates (EC50 = 0.28 mg/L).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.28 mg/L

Additional information

The acute toxicity of the substance was studied in a valid GLP test according to EU Method C.2 (key study). The test was performed under static conditions over a period of 48 hours. The tested daphnia (5 animals per concentration, 2 replicates) were exposed to the test substance at 5 different concentrations (0.056, 0.11, 0.23, 0.45, and 0.9 mg/L) for 48 h.

The highest concentration which did not cause immobilisation after 48 h was 0.1 mg/L (= EC0, arithmetic mean), whereas the concentration that caused 100% immobilisation after 48 h (= EC100, arithmetic mean) was 0.76 mg/L. The geometric mean between the EC0 and the EC100 value, which is considered to represent the EC50 value, was determined at 0.28 mg/L. In the control, no immobilisation occurred at all test concentrations after both exposure periods, ensuring the validity of the test system. The second required validity criterion for the test, a dissolved oxygen concentration > 3 mg/L at the end of the test in control and test vessels, was also fulfilled.

The second acute study with daphnia was probably conducted similar to OECD 202 but as only a result page is available and thus no details on the study are available the reliability of the study could not be assessed and therefore a reliability level of 4 (not assignable) is given to this study. The EC50 (geometric mean of EC0 and EC100) was determined to be 5.6 mg/L.

The effects of the test substance on marine invertebrates were not studied.