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EC number: 940-417-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-03-29 to 2012-04-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with no defieciencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 5,8,11,14,17-pentaoxahenicosane; 5,8,11,14-tetraoxaoctadecane
- EC Number:
- 940-417-3
- Molecular formula:
- C14H30O4 C16H34O5
- IUPAC Name:
- 5,8,11,14,17-pentaoxahenicosane; 5,8,11,14-tetraoxaoctadecane
- Details on test material:
- - Name of test material (as cited in study report): Polyglycol BB 300
- Physical state: Liquid, colourless
- Analytical purity: 99.6 % (a/a)
- Purity test date: 2011-06-28
- Lot/batch No.: DEG4155254
- Expiration date of the lot/batch: 2013-06-24
- Stability under test conditions: Not applicable
- Storage condition of test material: Room temperature, protected from light, in original container.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sewage treatment plant, D-31137 Hildesheim, Germany
- Pretreatment/Concentration of sludge: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition by aeration with Co2 free air until test start.
10 mL/L were used to initiate inoculation.
- Initial cell/biomass concentration: 6 * 10E9 CFU/L corresponding to 6 * 10E7 CFU/L in the test vessel - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 8 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium acc. to OECD 301 B / CO2 Evolution Test
- Test temperature: 22 +/- 2°C (20.0 - 23.0 °C, for 4 h down to 19.5 °C
- pH adjusted: no
- Continuous darkness: no, low light conditions (brown glass bottles)
TEST SYSTEM
- Culturing apparatus: 5000 mL brown glass flasks
- Number of culture flasks/concentration: 1 for the reference item, 1 for toxicity control (test and reference item), 2 for the control, 2 for the test item
- Method used to create aerobic conditions: Aeration with 30 - 100 mL/min
- Measuring equipment: Visual check of aeration twice per day
- Details of trap for CO2 and volatile organics if used: CO2 adsorption vessels were connected to the air outlets of the incubation vessels via a series of 3 gas wash bottles, each containing 100 mL of a 0.0125 mol/L Ba(OH)2 solution.
- Other: Application: The necessary amounts of bidistilled water, mineral salts medium and inoculum were placed in each of the incubation vessels. The vessels were aerated for 24 h with CO2 free air. After 24 h the CO2 adsorption vessels were connected to the air outlets of the incubation vessels. Test and reference item were weighed out and were transferred into the incubation vessels with bidistilled water. The vessels were made up to 3 L with bidistilled water and connected to the system for the production of CO2 free air.
SAMPLING
- Sampling frequency: Backtitration of the residual Ba(OH)2 with 0.05 N HCL was carried out three times a week during the first ten days and thereafter twice weekly.
- Sampling method: For each titration the first gas wash bottle was removed and a new bottle was connected to the last one.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Test medium without test and reference item
- Abiotic sterile control: No
- Toxicity control: Test item and reference item in test concentration
STATISTICAL METHODS:
The theoretical production of carbon dioxide (ThCO2) of the test item and functional control is calculated by the carbon content and the molecular formula, respectively.
The produced CO2 was calculated by: 1 mL HCl (c = 0.05 mol/L) = 1.1 mg CO2
The net amount of CO2 produced is calculated by correcting the results of the test item and functional control for endogenous CO2 production of the inoculum controls.
The biodegradation is calculated from the ratio theoretical CO2 production to net CO2 production.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20 mg/L
Results and discussion
- Preliminary study:
- Not performed
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 81
- Sampling time:
- 28 d
- Details on results:
- The adaptation phase of the functional control changed after 2 days into the degradation phase (degradation 10 %). The course of the degradation was rapid and the functional control reached the pass level of 60 % within 14 days and a biodegradation of 67 % after 28 days. The validity criterion degradation 60 % after 14 days is fulfilled.
The biodegradation of the test item is shown graphically in Figure 1 in comparison to the readily degradable functional control and the toxicity control. The mean 10 % level (beginning of biodegradation) was reached between day 8 and day 15 by all replicates. The 60 % pass level was reached within the 10-d-window between day 15 and day 19 by 3 replicates. 4 replicates reached the 60 % pass level between day 22 and day 27. Only on replicate did not reach the 60 % pass level and remained at 52 % biodegradation. The overall mean biodegradation after 28 days was 81 %.
The test item is classified as readily biodegradable after 28 days but without complying the 10-d-window.
In the inoculum control the total CO2 production was 26.9 mg CO2/L after 28 days.
BOD5 / COD results
- Results with reference substance:
- In the toxicity control containing both test and reference item a biodegradation rate of 38 % was determined after 7 days and it came to 90 % after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
Any other information on results incl. tables
CO2-Production and Biodegradation after 28 Days
CO2-Production |
Functional |
Test Item |
Toxicity Control |
|||||||
Control |
||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
|||
Net [mg/3 L] |
85.2 |
47.5 |
50.6 |
42.2 |
30.2 |
67.8 |
57.8 |
43.4 |
42.6 |
166.6 |
[mg/L] |
28.4 |
15.8 |
16.9 |
14.1 |
10.1 |
22.6 |
19.3 |
14.5 |
14.2 |
55.5 |
Theor. [mg/3 L] |
127.8 |
57.6 |
185.4 |
|||||||
[mg/L] |
42.6 |
19.2 |
61.8 |
|||||||
Degradation [%] |
67 |
82 |
88 |
73 |
52 |
100 |
100 |
75 |
74 |
90 |
CO2-Production and
Biodegradation in the Inoculum Control, Functional Control
and
Toxicity Control Samples
Study Day |
Date |
Inoculum |
Functional Control |
Toxicity Control |
||||
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
||||
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
||
4 |
2012-04-02 |
12.4 |
53.2 |
40.8 |
32 |
53.7 |
41.3 |
22 |
7 |
2012-04-05 |
12.1 |
33.9 |
62.6 |
49 |
42.1 |
71.3 |
38 |
12 |
2012-04-10 |
10.9 |
22.7 |
74.4 |
58 |
36.9 |
97.3 |
52 |
14 |
2012-04-12 |
5.7 |
9.0 |
77.7 |
61 |
25.0 |
116.6 |
63 |
18 |
2012-04-16 |
11.2 |
14.5 |
81.0 |
63 |
29.9 |
135.3 |
73 |
21 |
2012-04-19 |
9.1 |
7.4 |
81.0 |
63 |
19.6 |
145.8 |
79 |
25 |
2012-04-23 |
9.9 |
8.7 |
81.0 |
63 |
17.9 |
153.8 |
83 |
28 |
2012-04-26 |
2.8 |
6.3 |
84.5 |
66 |
13.4 |
164.4 |
89 |
29* |
2012-04-27 |
6.5 |
6.7 |
85.2 |
67 |
8.1 |
166.6 |
90 |
CO2-Production and
Biodegradation in the Inoculum Control and Test Item Samples
- Replicate 1 and 2
Study Day |
Date |
Inoculum |
Test Item |
|||||
Replicate 1 |
Replicate 2 |
|||||||
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
||||
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
||
4 |
2012-04-02 |
12.4 |
13.4 |
1.0 |
2 |
12.3 |
0.0 |
0 |
7 |
2012-04-05 |
12.1 |
14.5 |
3.4 |
6 |
13.1 |
1.0 |
2 |
12 |
2012-04-10 |
10.9 |
19.3 |
11.8 |
20 |
16.4 |
6.5 |
11 |
14 |
2012-04-12 |
5.7 |
11.0 |
17.1 |
30 |
22.1 |
22.9 |
40 |
18 |
2012-04-16 |
11.2 |
26.4 |
32.3 |
56 |
26.2 |
37.9 |
66 |
21 |
2012-04-19 |
9.1 |
10.8 |
34.0 |
59 |
13.3 |
42.1 |
73 |
25 |
2012-04-23 |
9.9 |
12.7 |
36.8 |
64 |
12.1 |
44.3 |
77 |
28 |
2012-04-26 |
2.8 |
11.3 |
45.3 |
79 |
8.7 |
50.2 |
87 |
29* |
2012-04-27 |
6.5 |
7.7 |
47.5 |
82 |
6.1 |
50.6 |
88 |
CO2-Production and
Biodegradation in the Inoculum Control and Test Item Samples
- Replicate 3 and 4
Study Day |
Date |
Inoculum |
Test Item |
|||||
Replicate 3 |
Replicate 4 |
|||||||
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
||||
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
||
4 |
2012-04-02 |
12.4 |
11.4 |
0.0 |
0 |
11.3 |
0.0 |
0 |
7 |
2012-04-05 |
12.1 |
9.7 |
0.0 |
0 |
9.0 |
0.4 |
0 |
12 |
2012-04-10 |
10.9 |
17.2 |
6.3 |
11 |
11.3 |
5.4 |
1 |
14 |
2012-04-12 |
5.7 |
21.0 |
21.6 |
38 |
10.7 |
16.1 |
9 |
18 |
2012-04-16 |
11.2 |
19.5 |
29.9 |
52 |
21.9 |
20.5 |
28 |
21 |
2012-04-19 |
9.1 |
12.0 |
32.8 |
57 |
13.5 |
23.7 |
36 |
25 |
2012-04-23 |
9.9 |
11.0 |
33.9 |
59 |
13.1 |
29.4 |
41 |
28 |
2012-04-26 |
2.8 |
9.0 |
40.1 |
70 |
8.5 |
30.2 |
51 |
29* |
2012-04-27 |
6.5 |
7.9 |
42.2 |
73 |
6.4 |
30.2 |
52 |
CO2-Production and
Biodegradation in the Inoculum Control and Test Item Samples
- Replicate 5 and 6
Study Day |
Date |
Inoculum |
Test Item |
|||||
Replicate 5 |
Replicate 6 |
|||||||
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
||||
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
||
4 |
2012-04-02 |
12.4 |
16.6 |
4.2 |
7 |
16.4 |
4.0 |
7 |
7 |
2012-04-05 |
12.1 |
13.3 |
5.4 |
9 |
13.3 |
5.2 |
9 |
12 |
2012-04-10 |
10.9 |
26.3 |
20.8 |
36 |
14.0 |
8.3 |
14 |
14 |
2012-04-12 |
5.7 |
18.8 |
33.9 |
59 |
13.8 |
16.4 |
28 |
18 |
2012-04-16 |
11.2 |
25.2 |
47.9 |
83 |
27.6 |
32.8 |
57 |
21 |
2012-04-19 |
9.1 |
14.9 |
53.7 |
93 |
18.6 |
42.3 |
73 |
25 |
2012-04-23 |
9.9 |
14.3 |
58.1 |
100 |
15.1 |
47.5 |
82 |
28 |
2012-04-26 |
2.8 |
11.9 |
67.2 |
100 |
10.9 |
55.6 |
97 |
29* |
2012-04-27 |
6.5 |
6.5 |
67.8 |
100 |
7.8 |
57.8 |
100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item is classified as readily biodegradable after 28 days but without complying the 10-d-window.
- Executive summary:
The ready biodegradability of the test item Polyglycol BB 300 (batch no.: DEG4155254) was determined with a non adapted activated sludge over a test period of 28 days in the Modified Sturm Test. The study was conducted from 2012-03-29 to 2012-04-27 according to OECD 301 B at Dr.U.Noack-Laboratorien. The test item was tested at a concentration of 8.0 mg/L with 8 replicates, corresponding to a carbon content (TOC) of 5.23 mg C/L in the test vessels. The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2 produced by the respiration of bacteria. The degradation was stopped on day 28 by acidification of the test solutions. The last titration was made on day 29, after residual CO2 had been purged from the test solutions over a period of 24 hours. The percentage CO2production was calculated in relation to the theoretical CO2production (ThCO2) of the test item. The biodegradation was calculated for each titration time.
To check the activity of the test system, sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % within 14 days and a biodegradation of 67 % after 28 days.
In the toxicity control containing both test and reference item a biodegradation rate of 38 % was determined after 7 days and it came to 90 % after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
The biodegradation of the test itemis shown graphically in comparison to the readily degradable functional control and the toxicity control. The mean 10 % level (beginning of biodegradation) was reached between day 8 and day 15 by all replicates. The 60 % pass level was reached within the 10-d-window between day 15 and day 19 by 3 replicates. 4 replicates reached the 60 % pass level between day 22 and day 27. Only one replicate did not reach the 60 % pass level and remained at 52 % biodegradation. The overall mean biodegradation after 28 days was 81 %.
The test item is classified as
readily biodegradableafter 28 days but without complying the 10-d-window.
Biodegradation of the Test Item Polyglycol BB 300 in Comparison
to the Functional Control and Toxicity ControlBiodegradation [%]
Study Day [d]
7
14
21
28
Test Item, 1st Replicate
6
30
59
82
Test Item, 2nd Replicate
2
40
73
88
Test Item, 3rd Replicate
0
38
57
73
Test Item, 4th Replicate
0
9
36
52
Test Item, 5th Replicate
9
59
93
100
Test Item, 6th Replicate
9
28
73
100
Test Item, 7th Replicate
0
24
57
75
Test Item, 8th Replicate
0
21
48
74
Functional Control
49
61
63
67
Toxicity Control
38
63
79
90
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