Chloroacetic anhydride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 February - 02 March 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Chloroacetic anhydride

EC Number:

208-794-2

EC Name:

Chloroacetic anhydride

Cas Number:

541-88-8

Molecular formula:

C4H4Cl2O3

IUPAC Name:

2-chloroacetyl 2-chloroacetate

Test material form:

other: dark brown solidified melt

Details on test material:

- Name of test material (as cited in study report): chloroacetic acid anhydride
- Physical state: solidified melt
- Analytical purity: 97.1 %
- Purity test date: 02 February 2007
- Lot/batch No.: 1028168
- Expiration date of the lot/batch: 08/2008
- Storage condition of test material: at 20°C, protected from light
- Other: pH 1.6

Test animals

Species:

rat

Strain:

other: Crl:WI(Han), SPF quality

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Laboratories, Research Models and Services, Germany GmbH, Sulzfeld, Germany,
via Biological Laboratory Services, Boehringer Ingelheim harma GmbH & Co. KG, Biberach, Germany
- Age at study initiation: approximately 9 weeks
- Weight at study initiation: males 224 g - 250 g, females 162 g - 171 g
- Fasting period before study: withdrawn in the afternoon of Day before administration (at about 16.00 h) over night. Immediately post administration, free access to food was allowed
again.
- Housing: barrier-protected system, cage (Noryl, type V).
- Diet (e.g. ad libitum): pelleted dry food, ad libitum
- Water (e.g. ad libitum): tap drinking water, ad libitum
- Acclimation period: 5- to 7-day

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C ± 2°C
- Humidity (%): 45% - 75%
- Air changes (per hr): minimum of 10 air changes per hour
- Photoperiod (hrs dark / hrs light): 12/12 hours

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Amount of vehicle (if gavage): 20 mL/kg
- Purity: demineralised

Doses:

50 mg/kg and 300 mg/kg
The administration volume was 20 mL/kg in each group.

No. of animals per sex per dose:

50 mg/kg: 3 males / 3 females
300 mg/kg: 3 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations: at arrival, during the study: at least twice daily and once on non-working days.
weighing: recorded one day before administration (Day -1), during observation period Day 1, Day 2, Day 8 and Day 15.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

other: approximate lethal dose (ALD)

Effect level:

>= 50 - <= 300 mg/kg bw

Based on:

test mat.

Mortality:

In rats, no mortality was observed subsequent to a single oral administration of 50 mg/kg.

At 300 mg/kg, female No. 251 was found dead on Day 1, 2.0 h post administration.
Immediately thereafter, rats No. 252 and No. 253 were sacrificed due to poor general condition for reasons of animal welfare

Clinical signs:

other: 50 mg/kg (males and females): Clinical signs were observed on Day 1 only and included prone position (0.25 h to 0.5 h post administration in males; at 1.0 h only in females), reduced motor activity (0.25 h to 6.0 h in males; at 2.0 h only in females) and

Body weight:

other body weight observations

Remarks:

No effects on body weight development were noted in all males and females treated with 50 mg/kg.

Gross pathology:

No gross macroscopic findings were noted at necropsy of animals administered 50 mg/kg.
At 300 mg//kg, necropsy findings in the three premature decedents comprised alterations of the stomach (discoloration of the wall in all 3
females) and the content of the colon (No. 251 and No. 252) .

Any other information on results incl. tables

At 50 mg/kg, no mortality occurred, no influence on body weight development and no necropsy findings were noted in both male and female rats. Clinical signs were observed on Day 1 only and included prone position, reduced motor activity and piloerection. Rats of both genders returned to normal on Day 2.

At 300 mg/kg, one female was found dead on Day 1, 2.0 h post administration. Immediately thereafter, the two remaining animals were sacrificed due to their poor general condition for reasons of animal welfare. Ante mortem, prone position and reduced motor activity, as well as reduced respiration rate were observed. Necropsy findings were recorded in all three female premature decedents and comprised alterations of the stomach and the content of the colon.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

Under the conditions of the present study, no mortality was seen in rats subsequent to oral administration of chloroacetic acid anhydride at doses of 50 mg/kg, but at 300 mg/kg, all females died or were sacrificed for reasons of animal welfare.
Thus, the approximate lethal dose (ALD) for chloroacetic acid anhydride is between 50 mg/kg to 300 mg/kg for male and female rats.