Bottom residues resulting from the synthesis of propene by propane dehydrogenation

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016-06-06 - 2016-06-08

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Hydrocarbons, C4-20, arom.-rich, alkene-manufg. byproduct

EC Number:

310-089-0

EC Name:

Hydrocarbons, C4-20, arom.-rich, alkene-manufg. byproduct

Cas Number:

102242-57-9

IUPAC Name:

102242-57-9

Details on test material:

- Name of test material (as cited in study report): Hydrocarbons, C4-20, arom.-rich, alkene-manufg. byproduct
- Substance type: organic
- Physical state: liquid
- Lot/batch No.: P0160-2015

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: P0160-2015
- Expiration date of the lot/batch: 22 Jan 2017


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test substance was stored in a freezer (<-18°C).
- Stability under test conditions: stable

Sampling and analysis

Analytical monitoring:

no

Remarks:

Analyses of the test substance preparations were not conducted, since a reliable method for analyses in the required concentration range could not be developed.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test substance is a mixture of sparingly water soluble components. Therefore test solutions were prepared separately for each test concentration as a water accommodated fraction (WAF) using a liquid-liquid saturator technique. For each test solution, a glass aspirator bottle (2-L) with a bottom side-outlet attached to a stopcock was filled with 2-L test media. Then test substance (considering density = 0.8018 g/cm³) was pipetted carefully on the water surface. The bottle was closed and the solution was stirred on a magnetic plate for about 2 days. The stirring was slow (approx. 100 rpm) so that no vortex forms. The undissolved test substance remained on the surface and the required volume of the WAF (1.2-L per test solution) was removed from the bottom port. The WAFs were visibly free of undissolved test substance.
Fresh test solutions were prepared and renewed daily and the test was performed in closed test vessels to maximize the exposure to all soluble components of the test substance.
All test solutions were visibly clear over the exposure period.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978.
From this date on this clone was cultured and bred continuously in the Ecotoxicology Laboratory of Experimental Toxicology and Ecology, BASF SE, Ludwigshafen Germany.
- Feeding during test: no
- Culture conditions: Daphnia brood stock are kept in mass cultures consisting of approx. 20 – 30 parthenogenetically reproducing females for a maximum of 4 weeks and fed live unicellular algae
(Desmodesmus subspicatus), cultured continuously at the test facility. All individuals in each mass culture originate from a single female and are thus genetic clones. After approximately
14 days the adults have produced at least 3 broods and the young can be used in tests. Offspring are removed from the mass cultures at least once daily during the normal work week
to ensure that young daphnia are <24-h old (first instar) at test initiation. Detailed records are kept (in test facility archives) to monitor the health of Daphnia brood stock cultures including
observations of young production, mortality, ephippia, and measurement of water chemistry parameters. Only young from healthy cultures without signs of stress are used for testing.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 – 3.20 mmol/L

Test temperature:

19.7 – 20.1°C (continuous monitoring)

pH:

7.9 - 8.1

Dissolved oxygen:

8.3 - 8.6 O2 value [mg/L]

Conductivity:

550 - 650 μS/cm

Nominal and measured concentrations:

loading rate: 0 (control), 2.2, 4.6, 10, 22, 46, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass flask (nominal volume 50 mL),
- Type: closed
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): The renewal period was 24 hours, via test water
- No. of organisms per vessel: 5 animals
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: approx. 0.1 animals/mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water (Elendt M4) OECD 202, is used as media for culture.
and test purposes
- Total organic carbon: <2 mg/L
- Ca/mg ratio: about 4 : 1
- Conductivity: 550 - 650 μS/cm
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: 234 - 694 lux at a wave length of 400 - 750 nm;

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- observation times: visually after 0, 24 and 48 h
- test parameter: swimming ability

RANGE-FINDING STUDY
- Test concentrations:In a preliminary range finding tes the 48 hour EC50 was between 1 and 10 mg/L. However in a first experiment there was only 20% mortality at 10 mg/L. Therefore 6 concentrations with a spacing factor of 2.2 were used for the second experiment.

Reference substance (positive control):

yes

Remarks:

sodium chloride

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

9.45 mg/L

Conc. based on:

other: based on loading rate:

Basis for effect:

mobility

Remarks on result:

not measured/tested

Results with reference substance (positive control):

- Results with reference substance valid? yes
This result is within the range of 3.88 – 7.22 g/L and indicates that the culture of Daphnia magna used in this study is responding normally to toxic stress.

The EC50(48h) of the reference substance sodium chloride was 4.99 g/L (experiment date: 08 Mar 2016, project number: 50E0789/12E060).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In a 48-hour acute toxicity study, neonate Daphnia magna were exposed to Hydrocarbons,C4-20, arom.-rich, alkene-manufg. byproduct at loading rates of 0 (control), 2.2, 4.6, 10, 22, 46, 100 mg/L under static-renewal conditions in accordance with the OECD 202 guideline. The water pH, temperature and dissolved oxygen were within acceptable guideline specifications. Immobilization (mortality) and sublethal effects were observed daily.
The 48 hour EC50 (EL50) value determined in this acute toxicity study was: 9.45 mg/L based on the loading rate.
The concentration of test substance in test media could not be verified analytically, therefore the effect concentrations are based on the loading rate used to prepare the test substance WAF solutions. According to guidance in OECD 23, for tests with chemicals that cannot be quantified by analytical methods at the concentrations causing effects, the effect concentration can be expressed based on the nominal concentrations or in the case of water accommodated fractions on the loading rate.