Benzaldehyde, 3-ethoxy-4-(.beta.-D-glucopyranosyloxy)-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July-September 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

April 13, 2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

10 mL of test samples from each replicates were drawn and mixed together for each group at 0 and 48 h. The samples were divided into two equal portions. One portion (20 mL) was sent for test concentration analysis and the second portion (20 mL) was stored at -20 ± 5 ºC temperature till the study completion. Active ingredient concentration in water was determined using the validated analytical method.

Vehicle:

yes

Remarks:

reconstituted water

Details on test solutions:

A quantity of 100.0 mg ethyl vanillin glucoside was transferred in 10 mL volumetric flask and volume was made up to the mark with reconstituted water to obtain the nominal concentration of 10 mg/mL. Volume of 1000 µL was taken from the stock, transferred using a micropipette and diluted to 100 mL with reconstituted water in glass beakers of 600 mL capacity to obtain the nominal test concentration of 100.0 mg ethyl vanillin glucoside/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

Healthy Daphnia magna (showing no signs of stress such as presence of males and ephippia, delay in the production of the first brood, discoloration, etc. and having normal behavior) less than 24 h old (second instar) cultured and bred in the Laboratory of Ecotoxicology, Jai Research Foundation, were used for the study. The master culture of Daphnia magna was originally procured from the MicroBio Test Inc, Kleimoer 15, 9030 Mariakerke (Gent), Belgium.

Gravid females were acclimatised to the test conditions for a minimum period of 48 h. During this period, Daphnia were fed with live algal cells (Pseudokirchneriella subcapitata). Freshly hatched daphnids belonging to the same stock (less than 24 h-old) were collected with the help of a micropipette and was used for the study. Feed was not provided during the exposure period.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

N/A

Post exposure observation period:

N/A

Hardness:

184.8 mg/L as CaCO3

Test temperature:

19.2-19.9 °C

pH:

7.33-7.45

Salinity:

N/A

Nominal and measured concentrations:

nominal 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

No immobilisation was observed at the test concentration of 100.0 mg ethyl vanillin glucoside/L as well as in the control (without ethyl vanillin glucoside) at 24 and 48 h. Daphnids exposed to 100.0 mg ethyl vanillin glucoside/L and control (without ethyl vanillin glucoside) did not exhibit any abnormal behavioural symptoms at 24 and 48 h.

Validity criteria fulfilled:

yes

Conclusions:

EC50 (24 and 48 h), NOEC and LOEC: Greater than the test concentration of 100.0 mg ethyl vanillin glucoside/L.

Executive summary:

The study was conducted as a limit study of 100.0 mg ethyl vanillin glucoside/L and no immobility was observed up to 48 h. Therefore, 24 and 48 h EC₅₀could not be calculated and considered to be greater than test concentration of 100.0 mg ethyl vanillin glucoside/L.

Description of key information

No toxic effects in Daphnia seen at the highest concentration tested 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L