Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 429-530-4 | CAS number: 96662-24-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 September 1998 to 11 September 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to recent EU test guidance in compliance with GLP.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Analysis of the only test concentration (8 mg/l) plus a control; additional analysis of the test concentration 8 mg/l without algal inoculum, at test start and after 72 hours of exposure.
- Vehicle:
- no
- Details on test solutions:
- To produce the only test concentration (8 mg/l), the test substance was weighed into water, treated in an ultrasonic bath for 1 hour and afterwards stirred for 24 hours on a magnetic stirrer. To remove undissolved particles, the resulting suspension was filtered using a folded filter of the pore size 7-12 um.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Species: Scenedesmus subspicatus CHODAT
Origin: Institute of Plant Physiology, University of Gottingen
Cultivation (stock cultures, precultures, test cultures): In a light chamber at 23 ± 2 °C and with a quantum flux which equals 120 μE/m-2s.
Water for dilution: deionised water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- Not reported.
- Test temperature:
- Not reported.
- pH:
- 8.2 - 10.3
- Dissolved oxygen:
- Not reported.
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- 8 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 300ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] Microcellcounter
The test solutions are incubated for a period of 72 hours, during which the cell density in each is measured at every 24 hours. The inhibition of growth and growth rate in relation to a control is determined after 72 hours of incubation. - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 3.75 mg/L
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 3.75 mg/L
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.75 mg/L
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.75 mg/L
- Basis for effect:
- growth rate
- Details on results:
- The above test result refers to a concentration of the test substance, which was calculated directly from analytically determined TOC values. According to the relevant product information, 1 mg/1 TOC equals to 1.5 mg/l of the test substance (structural formula: C22H19N502; molecular weight: 385.4 g/mole).
- Results with reference substance (positive control):
- Not applicable.
- Reported statistics and error estimates:
- Not reported.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No toxic effects against algae at 3.75 mg/l (maximum water solubility under exposure conditions).
- Executive summary:
Study data conducted to EU test guidance 92/69/EEC part C.3 and in compliance with GLP.
Study found no toxic effects against algae at 3.75 mg/l (maximum water solubility under exposure conditions).
Reference
Description of key information
No toxic effects against algae at 3.75 mg/l (maximum water solubility under exposure conditions).
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 3.75 mg/L
Additional information
Study data conducted to EU test guidance 92/69/EEC part C.3 and in compliance with GLP.
Study found no toxic effects against algae at 3.75 mg/l (maximum water solubility under exposure conditions).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
