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EC number: 221-416-0 | CAS number: 3088-31-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- The pollen tube growth test: A new alternative to the Draize eye irritation assay
- Author:
- UDO KRISTEN, UDO HOPPE, and WOLFGANG PAPE,
- Year:
- 1 993
- Bibliographic source:
- j. Soc.Cosmet.Chem.,44, 153-162 (1993)
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- The pollen tube growth test (PTG test) based on the growth response of in vitro growing tobacco pollen tubes were carried out for studiying ED50 value. the PTG test was among the five best of 30 in vitro alternatives to the Draize primary eye irritation test
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Sodium 2-(2-dodecyloxyethoxy)ethyl sulphate
- EC Number:
- 221-416-0
- EC Name:
- Sodium 2-(2-dodecyloxyethoxy)ethyl sulphate
- Cas Number:
- 3088-31-1
- Molecular formula:
- C16H34O6S.Na
- IUPAC Name:
- sodium 2-(2-dodecyloxyethoxy)ethyl sulphate
- Details on test material:
- - Name of test material (as cited in study report): sodium 2-(2-dodecyloxyethoxy) ethyl sulphate
- Substance type: Organic
- Physical state: liquid
Constituent 1
Test animals / tissue source
- Species:
- other: Nicotiana
- Strain:
- other: Nicotiana sylvestr
- Details on test animals or tissues and environmental conditions:
- Test Plant:
Tobacco plants of the species Nicotiana sylvestr (Spegazz& Comesvar. SCR) were cultivated from seeds in a greenhouse and set out to flower in the Botanical Garden of the University of Hamburg.The anthers of flowers that were not yet fully open were harvested and dried for two days at 20 deg C in a Petridish.The pollen was obtained by shaking the dried anthers and then freezing them in Eppendorf tubes (1.5 ml) at minus 18degC.
Growth Medium:
The culture mediumis composed of 10% saccharos(w/v), 0.01% boric acid( w/v), and 3 mM Ca(NO32) in double-distilled water.It is buffered w ith 10 mM MES( 2-[N-morpholino]-ethanesulfonaicc id) and adjusted to a pH of 5.6 with KOH.
Test system
- Vehicle:
- other: DMSO
- Controls:
- yes
- Amount / concentration applied:
- 10mM to 10-5 mM
- Duration of treatment / exposure:
- 18 hrs
- Observation period (in vivo):
- 18 hrs
Results and discussion
In vivo
Results
- Irritation parameter:
- other: ED50
- Time point:
- other: 18 hrs
- Score:
- 8.87
- Reversibility:
- not specified
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye
- Executive summary:
- Frozen pollen grain were resuspende in culture medium at a concentration of 1mg/ml. Test substances were serially diluted by a factor of 2. Dilutions were transferred the pollen suspension and incubated for18 h at 25 degC. Pollen tube cultures were washed twice with double distilled water and then stained with Alcian blue (0.05%) for 30 min. The dyed pollen tube walls were washed and finally destaine with citric acid (40%). The optical density was measured at 607nmEach concentration was tested in quadruplicates. The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eyeAccording to Kristen et.al.(1992). The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye
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