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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to fish:

Short term toxicity to fish for 96 h was evaluated for the test material . The test was performed as per EEC-Guideline L251.No stock solution was prepared.The test substance was mixed directly in the water of the tanks .10 fishes per concentration and control were used .Glass aquaria of 20 liters filled with 15 liters Dechlorinated tap water (carbon filter) was used as medium . The nominal test concentration used were 1.0, 1.8, 3.2, 5.8, 10 mg/l . The LC-50 values were calculated according to the maximum likelihood method, probit model. The lethal concentration (LC50) value of test material in zebra fish in a 96 hr study was found to be 6.3 mg/l. Based on the above effect concentration it can be concluded that test substance was toxic for fish and can be clasiffied as aquatic chronic 2 as per CLP criteria.

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test substance . On the basis of effects observed in a static fresh water system, the NOEC value for the substance is estimated to be4.12e-006mg/l for fish for 28 days of exposure duration.Based on the NOEC value it can be concluded that the test chemical is toxic to fish at environmentally relevant concentrations and can be considered aquatic cronic 1 as per the CLP classification criteria. 

Short term toxicity to aquatic invertebrate:

Short term toxicity of test material was evaluated on aquatic invertebrate Daphnia magna for 24 hr according to EEC-Guideline L251 .Cultures of daphnia were maintained in glass vessels containing approx. 2.5 1 of reconstituted waterat 20 ± 1"C (water is renewed partially thrice weekly). At each renewal the daphnia are fed a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h. In the pretest 24h before the start of the test reproductive daphnia were separated from the young by sieving all individuals through a 800 µm seive. This operation is repeated 6h before the start of the test and the young (6-24h of age) are retained for the test. Test was performed in beaker covered with watch glass having fill volume of 100 ml. The nominal concentrations at which test was performed were 0.0010, 0.0018, 0.0032, 0.0058, 0.010 0.018, 0.032, 0.058, 0.10, 0.18, 0.32 0.58, 1.0 mg/l. The EC50-values were calculated according to SPEARMAN-KAERBER in: D.J. Finney, 524-530, London (1964). EC-values were graphically determined on gausso-logarithmic probability paper. The effective concentration (EC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 24 hr study on the basis of mobility was estimated to be 0.023 mg/l. Based on the above effect concentration it can be considered that the test material is highly toxic to aquatic invertebrates and can be classified as per CLP criteria.

Long term toxicity to aquatic invertebrate:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on aquatic invertebrate was predicted for test substance . On the basis of effects observed in a static freshwater system, the NOEC value for the test substance is estimated to be1.79e-005mg/l for aquatic invertebrate for 21 days of exposure, Based on the NOEC value it can be concluded that the test chemical can be considered as toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered aquatic chronic 1 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Data available for the test chemicals has been reviewed to determine the toxicity to aqutic algae and cyanobacteria .The studies are as mentioned below:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance according to OECD Guideline 201. The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride, in Desmodesmus subspicatus was determined to be 0.13 mg/L. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic acute category 1 as per the CLP criteria.

Similarly the effect of test item was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 0.5mg/l, 1.25mg/l, 3.125mg/l, 7.813mg/l, 19.531mg/l and 48.828mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 0.610 mg/L.

Based on the EC50 value, test substancecan be considered astoxic to aquatic organisms and thus can be classified as aquatic acute category 1 as per the CLP criteria.

Toxicity to microorganism:

Toxicity studies to determine the inhibitory concentration of test material on the respiration of aerobic waste water bacteria were conducted according to EEC L 133, p. 118-122 (30. May 1988) guideline. The test was conducted on activated sludge from a sewage treatment plant for 3 hours at 20°C. The results were graphically determined . Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. 3,5-Dichlorophenol was used as referance substance. The effect concentration (IC50) was determined to be 15 mg/l , Test substance can be concluded to have moderate toxicity on microorganisms.

Additional information

Short term toxicity to fish:

Short term toxicity to fish for 96 h was evaluated for the test material . The test was performed as per EEC-Guideline L251.No stock solution was prepared.The test substance was mixed directly in the water of the tanks .10 fishes per concentration and control were used .Glass aquaria of 20 liters filled with 15 liters Dechlorinated tap water (carbon filter) was used as medium . The nominal test concentration used were 1.0, 1.8, 3.2, 5.8, 10 mg/l . The LC-50 values were calculated according to the maximum likelihood method, probit model. The lethal concentration (LC50) value of test material in zebra fish in a 96 hr study was found to be 6.3 mg/l. Based on the above effect concentration it can be concluded that test substance was toxic for fish and can be clasiffied as aquatic chronic 2 as per CLP criteria.

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test substance . On the basis of effects observed in a static fresh water system, the NOEC value for the substance is estimated to be4.12e-006mg/l for fish for 28 days of exposure duration.Based on the NOEC value it can be concluded that the test chemical is toxic to fish at environmentally relevant concentrations and can be considered aquatic cronic 1 as per the CLP classification criteria. 

Short term toxicity to aquatic invertebrate:

Short term toxicity of test material was evaluated on aquatic invertebrate Daphnia magna for 24 hr according to EEC-Guideline L251 .Cultures of daphnia were maintained in glass vessels containing approx. 2.5 1 of reconstituted waterat 20 ± 1"C (water is renewed partially thrice weekly). At each renewal the daphnia are fed a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h. In the pretest 24h before the start of the test reproductive daphnia were separated from the young by sieving all individuals through a 800 µm seive. This operation is repeated 6h before the start of the test and the young (6-24h of age) are retained for the test. Test was performed in beaker covered with watch glass having fill volume of 100 ml. The nominal concentrations at which test was performed were 0.0010, 0.0018, 0.0032, 0.0058, 0.010 0.018, 0.032, 0.058, 0.10, 0.18, 0.32 0.58, 1.0 mg/l. The EC50-values were calculated according to SPEARMAN-KAERBER in: D.J. Finney, 524-530, London (1964). EC-values were graphically determined on gausso-logarithmic probability paper. The effective concentration (EC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 24 hr study on the basis of mobility was estimated to be 0.023 mg/l. Based on the above effect concentration it can be considered that the test material is highly toxic to aquatic invertebrates and can be classified as per CLP criteria.

Long term toxicity to aquatic invertebrate:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on aquatic invertebrate was predicted for test substance . On the basis of effects observed in a static freshwater system, the NOEC value for the test substance is estimated to be1.79e-005mg/l for aquatic invertebrate for 21 days of exposure, Based on the NOEC value it can be concluded that the test chemical can be considered as toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered aquatic chronic 1 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Data available for the test chemicals has been reviewed to determine the toxicity to aqutic algae and cyanobacteria .The studies are as mentioned below:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance according to OECD Guideline 201. The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride, in Desmodesmus subspicatus was determined to be 0.13 mg/L. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic acute category 1 as per the CLP criteria.

Similarly the effect of test item was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 0.5mg/l, 1.25mg/l, 3.125mg/l, 7.813mg/l, 19.531mg/l and 48.828mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 0.610 mg/L.

Based on the EC50 value, test substancecan be considered astoxic to aquatic organisms and thus can be classified as aquatic acute category 1 as per the CLP criteria.

Toxicity to microorganism:

Toxicity of test material on microorganism was evaluated using two experimental reports described as follows:

In the first toxicity studies to determine the inhibitory concentration of test material on the respiration of aerobic waste water bacteria were conducted according to EEC L 133, p. 118-122 (30. May 1988) guideline. The test was conducted on activated sludge from a sewage treatment plant for 3 hours at 20°C. The results were graphically determined . Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. 3,5-Dichlorophenol was used as referance substance. The effect concentration (IC50) was determined to be 15 mg/l , Test substance can be concluded to have moderate toxicity on microorganisms.

Similarly in the second study Respiratory inhibition study ofactivated sludge by test material was conducted according to EC Directive 88/302/EEC - activated sludge: verificationof the inhibition of respiration;corresponds to the OECD Guidelines for Testing of Chemicals 209 and ISO Standard 8192.In the short-term respiration test, the effect of test substance on the respiration of aerobicMicroorganisms (activated sludge) after a short exposure time (30 to 180 min) were investigated.As the test result, the effective concentrations are determined, in which the breathinginhibited relative to a reference value without Priifsubstanz by 20, 50 and 80% (EC20,EC50, EC80).  Effect of the test substance on the respiration of municipal activated sludge were found asEC20: after 30 min about 40 mg / l ,EC50: - After 30 min> 1000 mg / l