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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Under the test conditions the percentage biodegradation of the test substance reached a mean of 6.5 % after 28 days based on its calculated ThODNH4. The test item can be considered to be not ready biodegradable. 

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The ready biodegradation of the test substance was inveatigated according to EU method C.4 -E, OECD guideline 301 D and OPPTS guideline 835.3110. Under the test conditions the percentage biodegradation of the test substance reached a mean of 6.5 % after 28 days based on its calculated ThODNH4. (The highest percentage biodegradation value, 8.5 % was observed on the 21st day based on ThODNH4.) The slight change was within the biological variability range of the applied test system. The test item can be considered to be not ready biodegradable.The measured quantities of nitrite and nitrate were below the LOQ (or were not detectable) in the measured samples throughout the study. The reference item Sodium benzoate was sufficiently degraded to a mean of 75.3 % after 14 days, and to a mean of 85.3 % after 28 days of incubation, based on ThODNH4. The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum.