Registration Dossier
Registration Dossier
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EC number: 203-026-2 | CAS number: 102-36-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well documented and scientifically acceptable
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 3,4-dichlorophenyl isocyanate
- EC Number:
- 203-026-2
- EC Name:
- 3,4-dichlorophenyl isocyanate
- Cas Number:
- 102-36-3
- Molecular formula:
- C7H3Cl2NO
- IUPAC Name:
- 1,2-dichloro-4-isocyanatobenzene
- Details on test material:
- purity: 99.94 %
Constituent 1
Method
- Target gene:
- no data
Species / strain
- Species / strain / cell type:
- Chinese hamster lung fibroblasts (V79)
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- 2, 10, 20 µg/ml in the absence of metabolic activation
5, 25, 50 µg/ml in the presence of S9-mix - Vehicle / solvent:
- no data
Results and discussion
Test results
- Key result
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 25 µg/ml without metanolic activation, 75 µg/ml with metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
Any other information on results incl. tables
3,4 -dichlorophenyl isocyanate does not induce chromosome mutations (=aberrations) in V79 chinese hamster cells, neither in the presence nor in the absence of a metabolic activation system, under the experimental conditions described
Applicant's summary and conclusion
- Executive summary:
method: cytogenetic assay in vitro with V79 chinese hamster cells (according OECD 473)
result: negative
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