4,4,5,5-Tetramethyl-1,3,2-dioxaborolane

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: acceptable, well-documented publication

Data source

Reference

Reference Type:

publication

Title:

Evaluation of a static renewal chronic toxicity test method for Daphnia magna Straus using boric acid

Author:

Gersich F M

Year:

1984

Bibliographic source:

Environmental Toxicoologie and Chemistry, 3: 98-94

Materials and methods

Principles of method if other than guideline:

chronic toxicity test (reproduction and mortality)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Boric acid

EC Number:

233-139-2

EC Name:

Boric acid

Cas Number:

10043-35-3

Molecular formula:

BH3O3

IUPAC Name:

boric acid

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Method of breeding:
- 20 °C
-photoperiod: 16 h light / 8 h dark
- food, water and glass changed twice a week
- Food type: algae (Selenastrum capricornutum)

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

19.5 - 20.5 °C

Dissolved oxygen:

7.3 - 8.0 mg/L

Nominal and measured concentrations:

test concentrations (nominal): 40, 80, 160, 320 and 600 mg/L
(analyzed concentrations ranged from 91.4 % to 106 % of the nominal concentrations)

Details on test conditions:

- Test vessels: 600-ml Pyrex beakers which contained a 1 .0 mm mesh stainless steel platform and five glass tubes (2.5 x 12 .5 cm) with 363 μm nylon mesh bottoms
- Each test beaker contained 500 ml of the appropriate amounts of test material, food and water.
- During the test the solutions were gently aerated to achieve 90 to 105 % saturation.
- There were four replicates for each test concentration and the control
- 20 organisms per concentration (five daphnids per replicate)
- The daphnids were fed S. capricornutum at an equivalent rate of 1 .25 mg/L dry weight
- Photoperiod of 16 h light / 8 h dark
- Three times a week the young produced by each adult were counted and discarded and adult survival was recorded
- After enumeration of the young, the adults were transferred to clean beakers containing fresh test and control solutions, in addition to a new supply of food

Results and discussion

Effect concentrations

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

299 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 244-382 mg/L

Details on results:

- There was no control mortality during the 21 d test.
- The maximum acceptable toxicant concentration (MATC) based on the endpoints of mean total young per replicate, meen brood size and mean size estimated to lie between 37 and 78 mg/L.