1,2,3,4-tetrahydro-1-naphthylamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

other information

Study period:

2005-11-16 to 2005-12-14 (experimental phase)

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,2,3,4-tetrahydro-1-naphthylamine

EC Number:

218-712-7

EC Name:

1,2,3,4-tetrahydro-1-naphthylamine

Cas Number:

2217-40-5

Molecular formula:

C10H13N

IUPAC Name:

1,2,3,4-tetrahydronaphthalen-1-amine

Details on test material:

- Batch: 7346-05167 Hauptlauf
- Purity: 98.2 area-% (BASF report No. 05L00169)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: STP Mannheim, Baden Wuerttemberg, Germany
- Storage conditions: Aeration in the laboratory until use
- Pretreatment: A suitable aliquot of the activated sludge was washed with tap water and sieved by a finely woven mesh with a mesh size about 1 mm and pre-aerated for about 24 hours. The sludge of this suspension was adjusted to a concentration of 6.0 g/L dry weight and then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance.
- Concentration of sludge: 30 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

20 mg/L

Based on:

DOC

Initial test substance concentration 2

Initial conc.:

25 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: According to guideline
- Test temperature: 22 ± 2°C
- Suspended solids concentration: 6 g/L

TEST SYSTEM
- Culturing apparatus: 2L conical flasks, filled up to a volume of 1000 mL
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: on days 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 27 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- Adsorption control: 1

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

< 10

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Degradation of reference substance after 14 days: 80 - 90%

Any other information on results incl. tables

- Elimination of the test substance by adsorption (% DOC): < 10 after 5 days

- Physico-chemical (abiotic) elimination of the test substance (% DOC): < 10 at the end of exposure

- Biodegradation degree in the inhibition control afier 14 days (% DOC): 40-50

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed