Canrenone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Oct - Nov 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Canrenone

EC Number:

213-554-5

EC Name:

Canrenone

Cas Number:

976-71-6

Molecular formula:

C22H28O3

IUPAC Name:

10,13-dimethyl-1,8,9,10,11,12,13,14,15,16-decahydro-3'H-spiro[cyclopenta[a]phenanthrene-17,2'-furan]-3,5'(2H,4'H)-dione

Details on test material:

- Name of test material (as cited in study report): Canrenone
- Analytical purity: 98.4%
- Lot/batch No.: 90515001

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

11 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL= 8.9-13.1 mg/L

Any other information on results incl. tables

Table 1: Cumulative immobilization (%) of Daphnia magna (n = 20) after exposure to canrenone

Dilution/measured concentration of canrenone (mg/L	24 hours	48 hours
Control (tap water)/0	0	0
1 :32 / 1,9	0	0
1 :16 / 2,5	0	5
1 :8 / 2,9	0	5
1 :4 / 5,9	0	10
1 :2 / 10,4	0	45
Saturated solution/16,6	45	90

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50/48 hours of canrenone to Daphnia magna was calculated to be 11.0 mg/L [95%
confidence limits: 8.9-13.1 mg/L] on the basis of measured concentrations (TOC). The highest
concentration with no effects was 1.9 mg/L.

Executive summary:

20 juvenile Daphnia magna were used for each test solution of canrenone and 20 for the tap water control. The Daphnia were exposed for aperiod of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. For the preparation of the test solutions a suspension with a nominal loading of 100 mgll was kept for approximately 30 min. in an ultrasonic bath and stirred for approximately 24 hours. This suspension was filtered through a glassfibre filter. The resulting solution served as the highest concentration (saturated solution). It was further diluted 1 :2, 1 :4, 1 :8, 1 :16 and 1 :32. Test concentrations were chosen on the basis of the results of a limit and dose-range finding test.

Sampies for the concentration analysis by TOC analysis were taken at 0 and 48 hours after start of exposure. The analytical results gave mean concentrations of 16.6 mg/L (saturated solution), 10.4 mg/L (1:2 solution), 5.9 mg/L (1:4 dilution), 2.9 mg/L (1:8 dilution), 2.5 mg/L (1 :16 dilution) and 1.9 mg/L (1 :32).

Immobilized Daphnia were observed after 48 hours in the dilutions 1 :16 (2.5 mglL) up to the saturated solution. It is likely that the immobilization is related to the test compound exposure.